Antibodies against prM protein distinguish between previous infection with dengue and Japanese encephalitis viruses

被引:144
作者
Cardosa, Mary Jane [1 ]
Wang, Seok Mui [1 ]
Sum, Magdline Sia H. [1 ]
Tio, Phaik Hooi [1 ]
机构
[1] Univ Malaysia Sarawak, Inst Hlth & Community Med, Kota Samarahan 94300, Sarawak, Malaysia
关键词
West Nile Virus; Dengue Virus; Japanese Encephalitis Virus; Dengue Haemorrhagic Fever; Japanese Encephalitis Virus;
D O I
10.1186/1471-2180-2-9
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Background: In Southeast Asia, dengue viruses often co-circulate with other flaviviruses such as Japanese encephalitis virus, and due to the presence of shared antigenic epitopes it is often difficult to use serological methods to distinguish between previous infections by these flaviviruses. Results: Convalescent sera from 69 individuals who were known to have had dengue or Japanese encephalitis virus infection were tested by western blotting against dengue, Japanese encephalitis and West Nile virus antigens. We determined that individuals who had been infected with dengue viruses had IgG responses against the premembrane protein of dengue viruses but not Japanese encephalitis, whereas individuals who had been infected with Japanese encephalitis had IgG specific for the premembrane protein of Japanese encephalitis virus but not the dengue viruses. None reacted with the premembrane protein of West Nile virus. Using the Pearson Chi Square test, it was determined that the difference between the two groups was highly significant with a p value of <0.001. Conclusion: The use of flavivirus premembrane protein in seroepidemiological studies will be useful in determining what flaviviruses have circulated in a community.
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页码:1 / 6
页数:6
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