Protective cellular immunity: Cytotoxic T-lymphocyte responses against dominant and recessive epitopes of influenza virus nucleoprotein induced by DNA immunization

被引：108

作者：

Fu, TM ^{[1
]}

Friedman, A ^{[1
]}

Ulmer, JB ^{[1
]}

Liu, MA ^{[1
]}

Donnelly, JJ ^{[1
]}

机构：

[1] MERCK RES LABS,DEPT VIRUS & CELL BIOL,W POINT,PA 19486

来源：

JOURNAL OF VIROLOGY | 1997年 / 71卷 / 04期

关键词：

D O I：

10.1128/JVI.71.4.2715-2721.1997

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

DNA immunization offers a novel means to induce cellular immunity in a population with a heterogeneous genetic background. An immunorecessive cytotoxic T-lymphocyte (CTL) epitope in influenza virus nucleoprotein (NP), residues 218 to 226, was identified when mice were immunized with a plasmid DNA encoding a full-length mutant NP in which the anchor residues for the immunodominant NP147-155 epitope were altered, Mice immunized with wild-type or mutant NP DNA were protected from lethal cross-strain virus challenge, and the protection could be adoptively transferred by immune splenocytes, indicating the role of cell-mediated immunity in the protection. DNA immunization is capable of eliciting protective cellular immunity against both immunodominant and immunorecessive CTL epitopes in the hierarchy seen with virus infection.

引用

页码：2715 / 2721

页数：7

共 36 条

[1] TRANSGENIC MICE LACKING CLASS-I MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED T-CELLS HAVE DELAYED VIRAL CLEARANCE AND INCREASED MORTALITY AFTER INFLUENZA-VIRUS CHALLENGE
BENDER, BS
CROGHAN, T
ZHANG, LP
SMALL, PA
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1992, 175 (04) : 1143 - 1145
[2] THE FOREIGN ANTIGEN-BINDING SITE AND T-CELL RECOGNITION REGIONS OF CLASS-I HISTOCOMPATIBILITY ANTIGENS
BJORKMAN, PJ
SAPER, MA
SAMRAOUI, B
BENNETT, WS
STROMINGER, JL
WILEY, DC
[J]. NATURE, 1987, 329 (6139) : 512 - 518
[3] Cao WX, 1996, J IMMUNOL, V157, P505
[4] PEPTIDE BINDING TO CLASS-I MHC ON LIVING CELLS AND QUANTITATION OF COMPLEXES REQUIRED FOR CTL LYSIS
CHRISTINCK, ER
LUSCHER, MA
BARBER, BH
WILLIAMS, DB
[J]. NATURE, 1991, 352 (6330) : 67 - 70
[5] BOVINE HERPESVIRUS-1 - IMMUNE-RESPONSES IN MICE AND CATTLE INJECTED WITH PLASMID DNA
COX, GJM
ZAMB, TJ
BABIUK, LA
[J]. JOURNAL OF VIROLOGY, 1993, 67 (09) : 5664 - 5667
[6] DOHERTY PC, 1992, ANNU REV IMMUNOL, V10, P123
[7] PRECLINICAL EFFICACY OF A PROTOTYPE DNA VACCINE - ENHANCED PROTECTION AGAINST ANTIGENIC DRIFT IN INFLUENZA-VIRUS
DONNELLY, JJ
FRIEDMAN, A
MARTINEZ, D
MONTGOMERY, DL
SHIVER, JW
MOTZEL, SL
ULMER, JB
LIU, MA
[J]. NATURE MEDICINE, 1995, 1 (06) : 583 - 587
[8] DONNELLY JJ, 1997, ANNU REV IMMUNOL, V60, P163
[9] CLEARANCE OF INFLUENZA-VIRUS RESPIRATORY-INFECTION IN MICE LACKING CLASS-I MAJOR HISTOCOMPATIBILITY COMPLEX-RESTRICTED CD8+ T-CELLS
EICHELBERGER, M
ALLAN, W
ZIJLSTRA, M
JAENISCH, R
DOHERTY, PC
[J]. JOURNAL OF EXPERIMENTAL MEDICINE, 1991, 174 (04) : 875 - 880
[10] EPSTEIN SL, 1993, J IMMUNOL, V150, P5484

← 1 2 3 4 →