Norovirus Diversity in Diarrheic Children from an African-Descendant Settlement in Belem, Northern Brazil

被引：30

作者：

Aragao, Glicelia Cruz ^{[1
]}

Pereira Mascarenhas, Joana D'Arc ^{[2
]}

Lima Kaiano, Jane Haruko ^{[3
]}

Sousa de Lucena, Maria Silvia ^{[2
]}

Monteiro Siqueira, Jones Anderson ^{[3
]}

Fumian, Tulio Machado ^{[2
]}

Hernandez, Juliana das Merces ^{[2
]}

de Oliveira, Consuelo Silva ^{[2
]}

Oliveira, Darleise de Souza ^{[2
]}

Araujo, Eliete da Cunha ^{[4
]}

Soares, Luana da Silva ^{[2
]}

Linhares, Alexandre Costa ^{[2
]}

Gabbay, Yvone Benchimol ^{[2
]}

机构：

[1] Univ Estado Para, Belem, Para, Brazil

[2] Inst Evandro Chagas, Ananindeua, Para, Brazil

[3] UFPA, Nucleo Med Trop, Belem, Para, Brazil

[4] UFPA, Inst Ciencias Saude, Belem, Para, Brazil

来源：

PLOS ONE | 2013年 / 8卷 / 02期

关键词：

ACUTE GASTROENTERITIS; MOLECULAR EPIDEMIOLOGY; HOSPITALIZED CHILDREN; GENETIC DIVERSITY; INFANTILE GASTROENTERITIS; ENZYME-IMMUNOASSAY; UNITED-STATES; OUTBREAKS; COMMUNITY; CALICIVIRUSES;

D O I：

10.1371/journal.pone.0056608

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Norovirus (NoV), sapovirus (SaV) and human astrovirus (HAstV) are viral pathogens that are associated with outbreaks and sporadic cases of gastroenteritis. However, little is known about the occurrence of these pathogens in relatively isolated communities, such as the remnants of African-descendant villages ("Quilombola"). The objective of this study was the frequency determination of these viruses in children under 10 years, with and without gastroenteritis, from a "Quilombola" Community, Northern Brazil. A total of 159 stool samples were obtained from April/2008 to July/2010 and tested by an enzyme immunoassay (EIA) and reverse transcription-polymerase chain reaction (RT-PCR) to detect NoV, SaV and HAstV, and further molecular characterization was performed. These viruses were detected only in the diarrheic group. NoV was the most frequent viral agent detected (19.7%-16/81), followed by SaV (2.5%-2/81) and HAstV (1.2%-1/81). Of the 16 NoV-positive samples, 14 were sequenced with primers targeting the B region of the polymerase (ORF1) and the D region of the capsid (ORF2). The results showed a broad genetic diversity of NoV, with 12 strains being classified as GII-4 (5-41.7%), GII-6 (3-25%), GII-7 (2-16.7%), GII-17 (1-8.3%) and GI-2 (1-8.3%), as based on the polymerase region; 12 samples were classified, based on the capsid region, as GII-4 (6-50%, being 3-2006b variant and 3-2010 variant), GII-6 (3-25%), GII-17 (2-16.7%) and GII-20 (1-8.3%). One NoV-strain showed dual genotype specificity, based on the polymerase and capsid region (GII-7/GII-20). This study provides, for the first time, epidemiological and molecular information on the circulation of NoV, SaV and HAstV in African-descendant communities in Northern Brazil and identifies NoV genotypes that were different from those detected previously in studies conducted in the urban area of Belem. It remains to be determined why a broader NoV diversity was observed in such a semi-isolated community.

引用

页数：7

共 53 条

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