Control of proliferation, endoreduplication and differentiation by the Arabidopsis E2Fa-DPa transcription factor

被引：320

作者：

De Veylder, L

Beeckman, T

Beemster, GTS

Engler, JD

Ormenese, S

Maes, S

Naudts, M

Van der Schueren, E

Jacqmard, A

Engler, G

Inzé, D

机构：

[1] Ghent Univ VIB, Dept Mol Genet, B-9000 Liege, Belgium

[2] Ghent Univ VIB, Dept Plant Genet, B-9000 Liege, Belgium

[3] Univ Ghent, INRA, B-9000 Ghent, Belgium

[4] Univ Liege, Lab Physiol Vegetale, B-4000 Liege, Belgium

来源：

EMBO JOURNAL | 2002年 / 21卷 / 06期

关键词：

Arabidopsis thaliana; cell cycle; cell differentiation; E2F-DP transcription; endoreduplication;

D O I：

10.1093/emboj/21.6.1360

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

New plant cells arise at the meristems, where they divide a few times before they leave the cell-cycle program and start to differentiate. Here we show that the E2Fa-DPa transcription factor of Arabidopsis thaliana is a key regulator determining the proliferative status of plant cells. Ectopic expression of E2Fa induced sustained cell proliferation in normally differentiated cotyledon and hypocotyl cells. The phenotype was enhanced strongly by the co-expression of E2Fa with its dimerization partner, DPa. In endoreduplicating cells, E2Fa-DPa also caused extra DNA replication that was correlated with transcriptional induction of S phase genes. Because E2Fa-DPa transgenic plants arrested early in development, we argue that controlled exit of the cell cycle is a prerequisite for normal plant development.

引用

页码：1360 / 1368

页数：9

共 50 条

[1] DcE2F, a functional plant E2F-like transcriptional activator from Daucus carota [J].