Genome-wide gene phylogeny of CIPK family in cassava and expression analysis of partial drought-induced genes

被引：56

作者：

Hu, Wei ^{[1
]}

Xia, Zhiqiang ^{[1
]}

Yan, Yan ^{[1
]}

Ding, Zehong ^{[1
]}

Tie, Weiwei ^{[1
]}

Wang, Lianzhe ^{[2
]}

Zou, Meiling ^{[1
]}

Wei, Yunxie ^{[1
]}

Lu, Cheng ^{[1
]}

Hou, Xiaowan ^{[1
]}

Wang, Wenquan ^{[1
]}

Peng, Ming ^{[1
]}

机构：

[1] Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou, Peoples R China

[2] Henan Univ Urban Construct, Coll Life Sci & Engn, Pingdingshan, Peoples R China

来源：

FRONTIERS IN PLANT SCIENCE | 2015年 / 6卷

关键词：

abiotic stress; cassava; CBL-interacting protein kinases; gene expression; genome-wide; identification; INTERACTING PROTEIN-KINASE; ABIOTIC STRESS TOLERANCE; CALCIUM SENSOR; SALT TOLERANCE; CONFERS SALT; FUNCTIONAL-CHARACTERIZATION; SIGNALING NETWORK; MOLECULAR-CLONING; ORYZA-SATIVA; CBL;

D O I：

10.3389/fpls.2015.00914

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Cassava is an important food and potential biofuel crop that is tolerant to multiple abiotic stressors. The mechanisms underlying these tolerances are currently less known. CBL-interacting protein kinases (CIPKs) have been shown to play crucial roles in plant developmental processes, hormone signaling transduction, and in the response to abiotic stress. However, no data is currently available about the CPK family in cassava. In this study, a total of 25 C/PK genes were identified from cassava genome based on our previous genome sequencing data. Phylogenetic analysis suggested that 25 MeCIPKs could be classified into four subfamilies, which was supported by exon-intron organizations and the architectures of conserved protein motifs. Transcriptomic analysis of a wild subspecies and two cultivated varieties showed that most MeCIPKs had different expression patterns between wild subspecies and cultivatars in different tissues or in response to drought stress. Some orthologous genes involved in CIPK interaction networks were identified between Arabidopsis and cassava. The interaction networks and co-expression patterns of these orthologous genes revealed that the crucial pathways controlled by CIPK networks may be involved in the differential response to drought stress in different accessions of cassava. Nine MeC1PK genes were selected to investigate their transcriptional response to various stimuli and the results showed the comprehensive response of the tested MeC1PK genes to osmotic, salt, cold, oxidative stressors, and ABA signaling. The identification and expression analysis of CIPK family suggested that C1PK genes are important components of development and multiple signal transduction pathways in cassava. The findings of this study will help lay a foundation for the functional characterization of the C1PK gene family and provide an improved understanding of abiotic stress responses and signaling transduction in cassava.

引用

页数：15

共 58 条

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