Bovine Staphylococcus aureus: Association of virulence genes, genotypes and clinical outcome

被引：120

作者：

Fournier, C. ^{[1
]}

Kuhnert, P. ^{[2
]}

Frey, J. ^{[2
]}

Miserez, R. ^{[3
]}

Kirchhofer, M. ^{[1
]}

Kaufmann, T. ^{[1
]}

Steiner, A. ^{[1
]}

Graber, H. U. ^{[1
]}

机构：

[1] Univ Bern, Fac Vet, Dept Clin Vet Med, Clin Ruminants, CH-3001 Bern, Switzerland

[2] Univ Bern, Fac Vet, Inst Vet Bacteriol, CH-3001 Bern, Switzerland

[3] Amt Lebensmittelsicherheit & Tiergesundheit, CH-7001 Chur, Switzerland

来源：

RESEARCH IN VETERINARY SCIENCE | 2008年 / 85卷 / 03期

关键词：

Staphylococcus aureus; Virulence factors; Contagiosity; Genotypes; PCR;

D O I：

10.1016/j.rvsc.2008.01.010

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Based on our clinical experience on bovine mastitis, we hypothesized that subtypes of Staphylococcus aureus (S. aureus) exist which differ in their contagious and pathogenic properties. In order to investigate this hypothesis, we analyzed strains of S. aureus isolated from spontaneous intramammary infection (IMI) with their virulence gene patterns and genotypes obtained by PCR amplification of the 16S-23S rRNA intergenic spacer (RS-PCR). The genotypes were then associated with epidemiological and clinical data including 26 herds. The results demonstrated a high association between genotypes and virulence gene patterns as well as between epidemiological and pathogenic properties of S. aureus. In particular, genotype B was related to high contagiosity and increased pathogenicity whereas the other types (C, OG) were found with infection of single cows. Because of the high clinical relevance, our results indicate the need to subtype the IMI-associated strains of S. aureus in the future. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：439 / 448

页数：10

共 38 条

[1] Toxin genes and other characteristics of Staphylococcus aureus isolates from milk of cows with mastitis [J].

Akineden, Ö ;

Annemüller, C ;

Hassan, AA ;

Lämmler, C ;

Wolter, W ;

Zschöck, M .

CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 2001, 8 (05) :959-964

[2] Genetic diversity and antimicrobial susceptibility profiles among mastitis-causing Staphylococcus aureus isolated from bovine milk samples [J].

Anderson, Kevin L. ;

Lyman, Roberta L. ;

Bodeis-Jones, Sonya M. ;

White, David G. .

AMERICAN JOURNAL OF VETERINARY RESEARCH, 2006, 67 (07) :1185-1191

[3] DETECTION OF STAPHYLOCOCCUS-AUREUS BY POLYMERASE CHAIN-REACTION AMPLIFICATION OF THE NUC GENE [J].

BRAKSTAD, OG ;

AASBAKK, K ;

MAELAND, JA .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (07) :1654-1660

[4] Immunity and mastitis - Some new ideas for an old disease [J].

Burton, JL ;

Erskine, RJ .

VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 2003, 19 (01) :1-+

[5] Enterotoxin production by Staphylococcus aureus isolated from mastitic cows [J].

Cenci-Goga, BT ;

Karama, M ;

Rossitto, PV ;

Morgante, RA ;

Cullor, JS .

JOURNAL OF FOOD PROTECTION, 2003, 66 (09) :1693-1696

[6]

Chang Byoung Sun, 2005, J Vet Sci, V6, P247

[7] Comparison of partial tuf gene sequences for the identification of lactobacilli [J].

Chavagnat, F ;

Haueter, M ;

Jimeno, J ;

Casey, MG .

FEMS MICROBIOLOGY LETTERS, 2002, 217 (02) :177-183

[8] Factors involved in the early pathogenesis of bovine Staphylococcus aureus mastitis with emphasis on bacterial adhesion and invasion -: A review [J].

Dego, OK ;

van Dijk, JE ;

Nederbragt, H .

VETERINARY QUARTERLY, 2002, 24 (04) :181-198

[9] Multilocus sequence typing for characterization of methicillin-resistant and methicillin-susceptible clones of Staphylococcus aureus [J].

Enright, MC ;

Day, NPJ ;

Davies, CE ;

Peacock, SJ ;

Spratt, BG .

JOURNAL OF CLINICAL MICROBIOLOGY, 2000, 38 (03) :1008-1015

[10] Characterization of a putative pathogenicity island from bovine Staphylococcus aureus encoding multiple superantigens [J].

Fitzgerald, JR ;

Monday, SR ;

Foster, TJ ;

Bohach, GA ;

Hartigan, PJ ;

Meaney, WJ ;

Smyth, CJ .

JOURNAL OF BACTERIOLOGY, 2001, 183 (01) :63-70

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