The art and design of genetic screens:: Drosophila melanogaster

被引：415

作者：

St Johnston, D

机构：

[1] Univ Cambridge, Wellcome CRC Inst, Cambridge CB2 1QR, England

[2] Univ Cambridge, Dept Genet, Cambridge CB2 1QR, England

来源：

NATURE REVIEWS GENETICS | 2002年 / 3卷 / 03期

基金：

英国惠康基金;

关键词：

D O I：

10.1038/nrg751

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The success of Drosophila melanogaster as a model organism is largely due to the power of forward genetic screens to identify the genes that are involved in a biological process. Traditional screens, such as the Nobel-prize-winning screen for embryonic-patterning mutants, can only identify the earliest phenotype of a mutation. This review describes the ingenious approaches that have been devised to circumvent this problem: modifier screens, for example, have been invaluable for elucidating signal-transduction pathways, whereas clonal screens now make it possible to screen for almost any phenotype in any cell at any stage of development.

引用

页码：176 / 188

页数：13

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