Beyond binding:: using phage display to select for structure, folding and enzymatic activity in proteins

被引：96

作者：

Forrer, P ^{[1
]}

Jung, S ^{[1
]}

Plückthun, A ^{[1
]}

机构：

[1] Univ Zurich, Inst Biochem, CH-8057 Zurich, Switzerland

来源：

CURRENT OPINION IN STRUCTURAL BIOLOGY | 1999年 / 9卷 / 04期

关键词：

D O I：

10.1016/S0959-440X(99)80073-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Phage display of a wide range of polypeptides has been increasingly used to identify novel molecules with useful binding properties for research, medical and industrial applications. Recent developments include methods for the selection of stabilized variants of a protein, the selection of regulatable enzymes and promising strategies for the selection and evolution of protein catalysts.

引用

页码：514 / 520

页数：7

共 55 条

[1] When blind is better: Protein design by evolution [J].

Arnold, FH .

NATURE BIOTECHNOLOGY, 1998, 16 (07) :617-618

[2] Directed evolution of biocatalysts [J].

Arnold, FH ;

Volkov, AA .

CURRENT OPINION IN CHEMICAL BIOLOGY, 1999, 3 (01) :54-59

[3] Phage display of a catalytic antibody to optimize affinity for transition-state analog binding [J].

Baca, M ;

Scanlan, TS ;

Stephenson, RC ;

Wells, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (19) :10063-10068

[4]

Bai C, 1997, METHOD ENZYMOL, V283, P141

[5] Immune versus natural selection: Antibody aldolases with enzymic rates but broader scope [J].

Barbas, CF ;

Heine, A ;

Zhong, GF ;

Hoffmann, T ;

Gramatikova, S ;

Bjornestedt, R ;

List, B ;

Anderson, J ;

Stura, EA ;

Wilson, IA ;

Lerner, RA .

SCIENCE, 1997, 278 (5346) :2085-2092

[6] Selection for a periplasmic factor improving phage display and functional periplasmic expression [J].

Bothmann, H ;

Plückthun, A .

NATURE BIOTECHNOLOGY, 1998, 16 (04) :376-380

[7] Minimizing a binding domain from protein A [J].

Braisted, AC ;

Wells, JA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) :5688-5692

[8] DNA shuffling of a family of genes from diverse species accelerates directed evolution [J].

Crameri, A ;

Raillard, SA ;

Bermudez, E ;

Stemmer, WPC .

NATURE, 1998, 391 (6664) :288-291

[9] A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage [J].

Demartis, S ;

Huber, A ;

Viti, F ;

Lozzi, L ;

Giovannoni, L ;

Neri, P ;

Winter, G ;

Neri, D .

JOURNAL OF MOLECULAR BIOLOGY, 1999, 286 (02) :617-633

[10]

DENG SJ, 1994, J BIOL CHEM, V269, P9533

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