Effect of Lycium barbarum polysaccharide on the improvement of antioxidant ability and DNA damage in NIDDM rats

被引:68
作者
Wu, Hao
Guo, Hongwei
Zhao, Rui
机构
[1] Fudan Univ, Sch Publ Hlth, Dept Nutr & Food Hyg, Shanghai 200032, Peoples R China
[2] Yanshan Univ, Coll Environm & Chem Engn, Dept Biol Engn, Qinhuangdao 066004, Peoples R China
来源
YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN | 2006年 / 126卷 / 05期
关键词
Lycium barbarum polysaccharide; oxidative stress; free radicals; non-insulin dependent diabetes mellitus; nitric oxide; DNA damage;
D O I
10.1248/yakushi.126.365
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
The effects of polysaccharide extracted from Lycium barbarum (LBP) on blood glucose, oxidative stress and DNA damage in rats with non-insulin dependent diabetes mellitus (NIDDM) were studied. The results show that LBP treatment (10 mg/kg (.) d) for 4 weeks led to decreased levels of blood glucose, malondialdehyde (MDA) and nitric oxide (NO) in serum of fasting rats; and to increased serum level of superoxidedismutase (SOD). Furthermore, LBP could reduce cellular DNA damage in peripheral lymphocytes of NIDDM rats. The DNA damage was determined by using the single cell gel (comet) assay with alkaline electrophoresis and was quantified by measuring tail length and tail moment. These results suggest that LBP can control blood glucose and modulate the metabolism of glucose, leading to significant improvement of oxidative stress markers (SOD, MDA) in rats with NIDDM. And that, LBP decreases DNA damage possibly via a decrease in oxidative stress levels. In conclusion, LBP as a dietary supplement may prevent the development of complications or even tendency to carcinogenesis in NIDDM rats.
引用
收藏
页码:365 / 371
页数:7
相关论文
共 46 条
[1]  
Akgul E, 1997, ATHEROSCLEROSIS, V130, P29
[2]  
Amos AF, 1997, DIABETIC MED, V14, pS7, DOI 10.1002/(SICI)1096-9136(199712)14:5+<S7::AID-DIA522>3.3.CO
[3]  
2-I
[4]   An examination of DNA strand breakage in the comet assay and antioxidant capacity in diabetic patients [J].
Anderson, D ;
Yu, TW ;
Wright, J ;
Ioannides, C .
MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 1998, 398 (1-2) :151-161
[5]  
BARRY H, 2002, FREE RADIC BIOL MED, V32, P968
[6]   APPARENT HYDROXYL RADICAL PRODUCTION BY PEROXYNITRITE - IMPLICATIONS FOR ENDOTHELIAL INJURY FROM NITRIC-OXIDE AND SUPEROXIDE [J].
BECKMAN, JS ;
BECKMAN, TW ;
CHEN, J ;
MARSHALL, PA ;
FREEMAN, BA .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (04) :1620-1624
[7]  
BISWAS SK, 1993, INDIAN J BIOCHEM BIO, V30, P293
[8]   Dietary fibre, glycaemic response, and diabetes [J].
Brennan, CS .
MOLECULAR NUTRITION & FOOD RESEARCH, 2005, 49 (06) :560-570
[9]   Diabetes mellitus: oxidative stress and wine [J].
Caimi, G ;
Carollo, C ;
Lo Presti, R .
CURRENT MEDICAL RESEARCH AND OPINION, 2003, 19 (07) :581-586
[10]   Glucose-induced oxidative stress in mesangial cells [J].
Catherwood, MA ;
Powell, LA ;
Anderson, P ;
McMaster, D ;
Sharpe, PC ;
Trimble, ER .
KIDNEY INTERNATIONAL, 2002, 61 (02) :599-608