Enhanced tolerance against salt-stress and freezing-stress of Escherichia coli cells expressing algal bbc1 gene

被引:22
作者
Tanaka, S
Ikeda, K
Miyasaka, H
机构
[1] Kansai Elect Power Co, Tech Res Ctr, Amagasaki, Hyogo 6610974, Japan
[2] Kansai Environm Engn Ctr Co Ltd, Chuo Ku, Osaka 5410052, Japan
关键词
D O I
10.1007/s002840010199
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The expression of the eukaryotic bbc1 (breast basic conserved) gene (the bbc1 gene of the marine green alga Chlamydomonas sp. W-80 strain) enhanced the tolerance against salt-stress and freezing-stress in E. coli cells, The expression of the BBC1 protein in the E. coli cells carrying the algal bbc1 gene and that in the Chlamydomonas W-80 cells were examined by Western blotting analysis. The result suggests that the eukaryotic BBC1 protein expressed in the E. coli cells has a protective function against the cellular dehydration.
引用
收藏
页码:173 / 177
页数:5
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