Leukocyte-endothelial adherence correlates with pancreatic nitric oxide production in early cerulein-induced pancreatitis in rats

被引:29
作者
Chen, HM
Shyr, MH
Lau, YT
Hwang, TL
Chen, MF
机构
[1] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Surg, Taipei, Taiwan
[2] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Anesthesia, Taipei, Taiwan
[3] Chang Gung Univ, Coll Med, Chang Gung Mem Hosp, Dept Physiol, Taipei, Taiwan
来源
SHOCK | 1998年 / 10卷 / 03期
关键词
D O I
10.1097/00024382-199809000-00011
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
The role of nitric oxide (NO) in microcirculation during the development of acute pancreatitis was not clear. An in vivo microscopic technique was used for evaluating leukocyte-endothelial adherence in the pancreatic microcirculation after induction (cerulein) of acute pancreatitis. Microdialysis was performed to detect pancreatic nitrate concentration (NO level) by high-performance liquid chromatography. Cerulein caused significantly reduced flow velocity in 1 h (31%) and increased the number of sticking leukocytes in 2 h; both persisted for at least 3 h, Pancreatic NO level was found to be significantly elevated (2.5-fold) in 1 h and also persisted for 3 h, Both microcirculatory changes and NO elevation were significantly alleviated in cerulein-induced animals pretreated with NO synthase inhibitor (N-G-nitro-L-arginine), indicating that elevation of NO could precede and account for a major portion of the observed microcirculatory changes. Furthermore, there was a strong positive correlation between numbers of adherent leukocytes and pancreatic NO level, suggesting that during the development of acute pancreatitis, NO could play an adverse role in microcirculation.
引用
收藏
页码:218 / 222
页数:5
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