A rapid and sensitive LC-MS/MS method for the determination of Pulsatilla saponin D in rat plasma and its application in a rat pharmacokinetic and bioavailability study

被引：16

作者：

Ouyang, Hui ^{[1
,2
]}

Guo, Yicheng ^{[1
]}

He, Mingzhen ^{[1
]}

Zhang, Jinlian ^{[1
]}

Huang, Xiaofang ^{[1
]}

Zhou, Xin ^{[3
]}

Jiang, Hongliang ^{[4
]}

Feng, Yulin ^{[1
]}

Yang, Shilin ^{[1
,2
]}

机构：

[1] Jiangxi Univ Tradit Chinese Med, Nanchang 330006, Peoples R China

[2] Guangzhou Univ Chinese Med, Guangzhou 510405, Guangdong, Peoples R China

[3] Guizhou Normal Univ, Res Ctr Qual Control Nat Med, Guiyang 550001, Peoples R China

[4] Huazhong Univ Sci & Technol, Tongji Sch Pharm, Wuhan 430030, Peoples R China

来源：

BIOMEDICAL CHROMATOGRAPHY | 2015年 / 29卷 / 03期

关键词：

Pulsatilla saponin D; LC-MS/MS; pharmacokinetics; bioavailability; TRITERPENOID SAPONINS; KOREANA;

D O I：

10.1002/bmc.3286

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

A simple, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of Pulsatilla saponin D, a potential antitumor constituent isolated from Pulsatilla chinensis in rat plasma. Rat plasma samples were pretreated by protein precipitation with methanol. The method validation was performed in accordance with US Food and Drug Administration guidelines and the results met the acceptance criteria. The method was successfully applied to assess the pharmacokinetics and oral bioavailability of Pulsatilla saponin D in rats. Copyright (c) 2014 John Wiley & Sons, Ltd.

引用

页码：373 / 378

页数：6

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