Consecutive in vivo measurement of nitric oxide in transient forebrain ischemic rat under normothermia and hypothermia

被引：19

作者：

Sugimura, T ^{[1
]}

Sako, K ^{[1
]}

Tohyama, Y ^{[1
]}

Yonemasu, Y ^{[1
]}

机构：

[1] Asahikawa Med Coll, Dept Neurosurg, Asahikawa, Hokkaido 078, Japan

来源：

BRAIN RESEARCH | 1998年 / 808卷 / 02期

关键词：

cerebral ischemia; cerebral blood flow; hypothermia; microdialysis; nitric oxide; rat;

D O I：

10.1016/S0006-8993(98)00822-1

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The effects of hypothermia on production of nitric oxide (NO) in ischemic brain were investigated by using in vivo microdialysis. Male Wistar rats were randomly divided into three groups; saline-treated normothermic group (37 degrees C, n = 6), 30 mg/kg N-nitro-L-auginine methyl ester(L-NAME)-treated normothermic group (n = 6), and saline-treated hypothermic group (30 degrees C, n = 6). Transient forebrain ischemia was produced by bilateral common carotid artery occlusion combined with hypotension (MABP = 50 mmHg). Saline-treated normothermic animals resulted in a reduction of LCBF to 9% of baseline. Saline-treated hypothermic rats revealed the similar changes of LCBF. In contrast, L-NAME administration reduced the basal CBF to 85% of saline-treated group and to 8% after ischemia. NO products were decreased during ischemia and transiently increased after reperfusion in saline-treated groups. However, the increase of NO products after reperfusion was less significant in the hypothermia. L-NAME-treated group showed a constant reduction of NO production during ischemia and after reperfusion. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：313 / 316

页数：4

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