Bioengineered human bone tissue using autogenous osteoblasts cultured on different biomatrices

被引:53
作者
Hofmann, A [1 ]
Konrad, L
Gotzen, L
Printz, H
Ramaswamy, A
Hofmann, C
机构
[1] Univ Marburg, Dept Traumatol, Marburg, Germany
[2] Johannes Gutenberg Univ Mainz, Dept Traumatol, D-6500 Mainz, Germany
[3] Univ Marburg, Dept Urol, Marburg, Germany
[4] Univ Marburg, Dept Internal Med & Gastroenterol, Marburg, Germany
[5] Univ Marburg, Dept Pathol, Marburg, Germany
关键词
bone; tissue engineering; bone grafting; osteoblast; perfusion culture;
D O I
10.1002/jbm.a.10594
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Surgical treatment of critical-size posttraumatic bone defects is still a challenging problem, even in modern bone and joint surgery. Progress in cellular and molecular biology during the last decade now permits novel approaches in bone engineering. Recent conceptual and technical advances have enabled the use of mitotically expanded, bone-derived cells as a therapeutic approach for tissue repair. Using three different tissue carrier systems, we successfully cultivated human osteoblasts in a newly developed perfusion. chamber. We studied cell proliferation and the expression of osteocalcin, osteopontin, bone morphogenetic protein-2A, alkaline phosphatase, and vascular endothelial growth factor as parameters for osteoblast function and viability. Adherence of highly enriched human osteoblasts had already started after I h and resulted in completely overgrown human bone pieces after 10 days. Expression analysis of bone-specific alkaline phosphatase indicated differentiating osteoblasts, whereas the high mRNA expression of osteocalcin and osteopontin revealed terminally differentiated osteoblasts and the process of mineralization. Additionally, gene expression was significantly higher when demineralized bone was used as biomatrix, compared to autoclaved bone and hydroxyapatite ceramics. We conclude that with our newly developed perfusion culture system, vital autogenous bone implants of clinically applicable size can be generated within 17 days in order to manage critical-size bone defects. (C) 2003 Wiley Periodicals, Inc.
引用
收藏
页码:191 / 199
页数:9
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