Atomic-scale dynamical structures of fatty acid bilayers observed by ultrafast electron crystallography

The structure and dynamics of a biological model bilayer are reported with atomic-scale resolution by using ultrafast electron crystallography. The bilayer was deposited as a Langmuir-Blodgett structure of arachiclic (eicosanoic) fatty acids with the two chains containing 40 carbon atoms (approximate to 50 angstrom), on a hydrophobic substrate, the hydrogen terminated silicon(l 11) surface. We determined the structure of the 2D assembly, establishing the orientation of the chains and the subunit cell of the CH2 distances: a(0) = 4.7 angstrom, b(0) = 8.0 angstrom, and c(0) = 2.54 angstrom. For structural dynamics, the diffraction frames were taken every 1 picosecond after a femtosecond temperature jump. The observed motions, with sub-A resolution and monolayer sensitivity, clearly indicate the coherent anisotropic expansion of the bilayer solely along the aliphatic chains, followed by nonequilibrium contraction and restructuring at longer times. This motion is indicative of a nonlinear behavior among the anharmonically coupled bonds on the ultrashort time scale and energy redistribution and diffusion on the longer time scale. The ability to observe such atomic motions of complex structures and at interfaces is a significant leap forward for the determination of macromolecular dynamical structures by using ultrafast electron crystallography.