Changes in paw oedema triggered via bradykinin B1 and B2 receptors in streptozotocin-diabetic rats

The present study investigated hind paw oedema mediated by bradykinin B-1 and B-2 receptors in streptozotocin-diabetic rats. Paw oedema induced by intraplantar (i.pl.) injection of bradykinin or the selective bradykinin B-2 receptor agonist, Tyrosines-bradykinin ([Tyr(8)]bradykinin) (both 3 nmol/paw), was significantly reduced at 4 weeks after streptozotocin treatment (34 +/- 8% and 40 +/- 7%). At 6 weeks after streptozotocin, when paw oedema caused by substance P or prostaglandin P-2 (both 10 nmol/paw) was unchanged, inhibition of bradykinin B-2 receptor-mediated oedema was maximal (66 +/- 6% and 72 +/- 2%, for bradykinin and [Tyr(8)]bradykinin, respectively). The selective bradykinin B-1 receptor agonist, [des-Arg(9)]bradykinin (100 nmol/paw), induced only slight paw oedema in non-diabetic controls. Responses to [des-Arg(9)]bradykinin were markedly enhanced 8 weeks after streptozotocin (from 0.09 +/- 0.01 to 0.38 +/- 0.05 ml), less so at 10 weeks (0.22 +/- 0.03 ml), and returning to basal values at 12 weeks (0.11 +/- 0.03 ml). Treatment with insulin protamine zinc (1-3 U/day/7 weeks, s.c.) did not reverse the inhibition of responses to [Tyr(8)]bradykinin or the potentiation of responses to [des-Arg(9)]bradykinin seen at 8 weeks. Thus, streptozotocin-induced diabetes induces long-lasting alterations in oedematogenic responsiveness to kinins in the rat, characterized by marked reduction of oedema involving activation of bradykinin B-2 receptors, associated with enhancement of bradykinin B-1 receptor-mediated oedema. (C) 2001 Published by Elsevier Science B.V.