Endogenous skin fluorescence includes bands that may serve as quantitative markers of aging and photoaging

被引:156
作者
Kollias, N [1 ]
Gillies, R [1 ]
Moran, M [1 ]
Kochevar, IE [1 ]
Anderson, RR [1 ]
机构
[1] Harvard Univ, Massachusetts Gen Hosp, Sch Med, Wellman Labs Photomed, Boston, MA 02114 USA
关键词
spectroscopy; UVB;
D O I
10.1046/j.1523-1747.1998.00377.x
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Aging and photoaging cause distinct changes in skin cells and extracellular matrix. Changes in hairless mouse skin as a function of age and chronic UVB exposure were investigated by fluorescence excitation spectroscopy, Fluorescence excitation spectra were measured in vivo, on heat-separated epidermis and dermis, and on extracts of mouse skin to characterize the absorption spectra of the emitting chromophores. Fluorescence excitation spectra obtained in vitro on 6 wk old mouse skin had maxima at 295, 340, and 360 nm; the 295 nm band was the dominant band. Using heat separated tissue, the 295 nm band predominantly originated in the epidermis and the bands at 340 and 360 nm originated in the dermis, The 295 nm band was assigned to tryptophan fluorescence, the 340 nm band to pepsin digestable collagen cross-links fluorescence and the 360 nm band to collagenase digestable collagen cross-links fluorescence. Fluorescence excitation maxima remained unchanged in chronologically aged mice (34-38 wk old), whereas the 295 nm band decreased in intensity with age and the 340 nm band increased in intensity with age. In contrast, fluorescence excitation spectra of chronically UVB exposed mice showed a large increase in the 295 nm band compared with age-matched controls and the bands at 340 and 350 nm were no longer distinct. Two new bands appeared in the chronically exposed mice at 270 nm and at 305 nm, These reproducible changes in skin autofluorescence suggest that aging causes predictable alterations in both epidermal and dermal fluorescence, whereas chronic UV exposure induces the appearance of new fluorphores.
引用
收藏
页码:776 / 780
页数:5
相关论文
共 17 条
[1]  
ANDERSON RR, 1989, ARCH DERMATOL, V125, P999, DOI 10.1001/archderm.125.7.999
[2]   THE DIFFUSION OF WATER ACROSS THE STRATUM-CORNEUM AS A FUNCTION OF ITS WATER-CONTENT [J].
BLANK, IH ;
MOLONEY, J ;
EMSLIE, AG ;
SIMON, I ;
APT, C .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1984, 82 (02) :188-194
[3]  
CORDEIRO PG, 1994, PLAST RECONSTR SURG, V96, P673
[4]   A FLUORESCENT COMPOUND IN COLLAGEN AND ITS RELATION TO AGE OF ANIMAL [J].
DEYL, Z ;
SULCOVA, H ;
PRAUS, R ;
GOLDMAN, JN .
EXPERIMENTAL GERONTOLOGY, 1970, 5 (01) :57-&
[5]   EFFECTS OF SYSTEMIC INDOMETHACIN, MECLIZINE, AND BW755C ON CHRONIC ULTRAVIOLET-B-INDUCED EFFECTS IN HAIRLESS MOUSE SKIN [J].
KOCHEVAR, IE ;
MORAN, M ;
LYON, N ;
FLOTTE, T ;
SIEBERT, E ;
GANGE, RW .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1993, 100 (02) :186-193
[6]   Fluorescence photography in the evaluation of hyperpigmentation in photodamaged skin [J].
Kollias, N ;
Gillies, R ;
CohenGoihman, C ;
Phillips, SB ;
Muccini, JA ;
Stiller, MJ ;
Drake, LA .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1997, 36 (02) :226-230
[7]  
Lakowicz JR., 1983, Principles of Fluorescence Spectroscopy, P187, DOI [10.1007/978-1-4615-7658-7_7, DOI 10.1007/978-1-4615-7658-7_7]
[8]   INVIVO FLUORESCENCE OF HUMAN-SKIN - A POTENTIAL MARKER OF PHOTOAGING [J].
LEFFELL, DJ ;
STETZ, ML ;
MILSTONE, LM ;
DECKELBAUM, LI .
ARCHIVES OF DERMATOLOGY, 1988, 124 (10) :1514-1518
[9]   INSITU DIFFERENTIATION BETWEEN NEVI AND MALIGNANT MELANOMAS BY FLUORESCENCE MEASUREMENTS [J].
LOHMANN, W ;
NILLES, M ;
BODEKER, RH .
NATURWISSENSCHAFTEN, 1991, 78 (10) :456-457
[10]   Fluorescence photography in the evaluation of acne [J].
Lucchina, LC ;
Kollias, N ;
Gillies, R ;
Phillips, SB ;
Muccini, JA ;
Stiller, MJ ;
Trancik, RJ ;
Drake, LA .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1996, 35 (01) :58-63