Development and characterisation of a large diameter decellularised vascular allograft

被引:11
作者
Aldridge, A. [1 ]
Desai, A. [2 ]
Owston, H. [1 ]
Jennings, L. M. [2 ]
Fisher, J. [2 ]
Rooney, P. [3 ]
Kearney, J. N. [3 ]
Ingham, E. [1 ]
Wilshaw, S. P. [1 ]
机构
[1] Univ Leeds, Inst Med & Biol Engn, Sch Biomed Sci, Leeds LS2 9JT, W Yorkshire, England
[2] Univ Leeds, Inst Med & Biol Engn, Sch Mech Engn, Leeds LS2 9JT, W Yorkshire, England
[3] NHS Blood & Transplant Tissue & Eye Serv, 14 Estuary Banks,Estuary Commerce Pk, Liverpool L24 8RB, Merseyside, England
基金
英国工程与自然科学研究理事会; 英国惠康基金;
关键词
Decellularisation; Acellular; Scaffold; Aorta; VENTRICULAR OUTFLOW TRACT; AORTIC-VALVE ALLOGRAFTS; ROOT REPLACEMENT; PULMONARY VALVE; IN-VIVO; RECONSTRUCTION; HOMOGRAFT; CALCIFICATION; IMMUNOREACTIVITY; TRANSPLANTATION;
D O I
10.1007/s10561-017-9673-y
中图分类号
Q2 [细胞生物学];
学科分类号
071013 [干细胞生物学];
摘要
The aims of this study were to develop a biological large diameter vascular graft by decellularisation of native human aorta to remove the immunogenic cells whilst retaining the essential biomechanical, and biochemical properties for the ultimate benefit of patients with infected synthetic grafts. Donor aortas (n = 6) were subjected to an adaptation of a propriety decellularisation process to remove the cells and acellularity assessed by histological analysis and extraction and quantification of total DNA. The biocompatibility of the acellular aortas was determined using standard contact cytotoxicity tests. Collagen and denatured collagen content of aortas was determined and immunohistochemistry was used to determine the presence of specific extracellular matrix proteins. Donor aortas (n = 6) were divided into two, with one half subject to decellularisation and the other half retained as native tissue. The native and decellularised aorta sections were then subject to uniaxial tensile testing to failure [axial and circumferential directions] and suture retention testing. The data was compared using a paired t-test. Histological evaluation showed an absence of cells in the treated aortas and retention of histoarchitecture including elastin content. The decellularised aortas had less than 15 ng mg(-1) total DNA per dry weight (mean 94% reduction) and were biocompatible as determined by in vitro contact cytotoxicity tests. There were no gross changes in the histoarchitecture [elastin and collagen matrix] of the acellular aortas compared to native controls. The decellularisation process also reduced calcium deposits within the tissue. The uniaxial tensile and suture retention testing revealed no significant differences in the material properties (p > 0.05) of decellularised aorta. The decellularisation procedure resulted in minimal changes to the biological and biomechanical properties of the donor aortas. Acellular donor aorta has excellent potential for use as a large diameter vascular graft.
引用
收藏
页码:287 / 300
页数:14
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