Control of bacterial aggregation by thermoresponsive glycopolymers

被引:143
作者
Pasparakis, George [1 ]
Cockayne, Alan [1 ]
Alexander, Cameron [1 ]
机构
[1] Univ Nottingham, Sch Pharm, Ctr Biomol Sci, Inst Infect Immun & Inflammat, Nottingham NG7 RD, England
基金
英国工程与自然科学研究理事会;
关键词
D O I
10.1021/ja074349z
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Thermo responsive polymers with pendant glucose functionality form aggregates with bacteria cells that can be assembled/disassembled reversibly through temperature cycling. The polymers are chain extended at temperatures below 40 degrees C and display glucose moieties to solution, whereas above this temperature the globular state of the polymer "hides" the sugar groups. The specificity and reversibility of the glucose display is demonstrated chemically via a boronic acid-alizarin coupled assay and through cell aggregation assays utilizing bacteria with and without fim H sugar recognition sites.
引用
收藏
页码:11014 / +
页数:3
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