Development of by time-dependent a liquid phase immunoassay measurements of the transient magneto-optical birefringence using functionalized magnetic nanoparticles

被引：24

作者：

Glöckl, G

Brinkmeier, V

Aurich, K

Romanus, E

Weber, P

Weitschies, W

机构：

[1] Univ Greifswald, Inst Pharm, D-17487 Greifswald, Germany

[2] Univ Jena, Inst Festkorperphys, D-07743 Jena, Germany

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2005年 / 289卷

关键词：

field-induced magnetic birefringence; brown relaxation; specifically binding MNP; prostate specific antigen; human IgG;

D O I：

10.1016/j.jmmm.2004.11.135

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The transient magneto-optical birefringence was investigated as a new liquid phase immunoassay technique. Specifically binding magnetic probes using monoclonal as well as polyclonal antibodies were investigated. The detection limit for optimized magnetic nanoparticles was determined. Binding experiments of polyclonal probes with their specific antigen yielded increased particle sizes as determined from magneto-optical relaxation curves. Aggregation of magnetic probes due to cross-linking caused gradually decreased signal intensity. (c) 2004 Elsevier B.V. All rights reserved.

引用

页码：480 / 483

页数：4

共 6 条

[1] Nanoparticle composition of a ferrofluid and its effects on the magnetic properties [J].