Interaction of human T-cell lymphotropic virus type I Rex protein with Dicer suppresses RNAi silencing

被引：27

作者：

Abe, Makoto ^{[1
]}

Suzuki, Hitoshi ^{[1
]}

Nishitsuji, Hironori ^{[1
]}

Shida, Hisatoshi ^{[3
]}

Takaku, Hiroshi ^{[1
,2
]}

机构：

[1] Chiba Inst Technol, Dept Life & Environm Sci, Chiba 2750016, Japan

[2] Chiba Inst Technol, High Technol Res Ctr, Chiba 2750016, Japan

[3] Hokkaido Univ, Inst Med Genet, Dept Mol Virol, Kita Ku, Sapporo, Hokkaido 0600815, Japan

来源：

FEBS LETTERS | 2010年 / 584卷 / 20期

关键词：

HTVL-1; Rex; Dicer; HTLV-1; mRNA; Rex-response element (RxRE); RNAi; HTLV-1 Rex-Dicer complex; NUCLEAR EXPORT SIGNAL; HEPATITIS-C VIRUS; HTLV-1; TAX; SEQUENCE REQUIREMENTS; RESPONSE ELEMENT; CORE PROTEIN; VIRAL-RNA; LEUKEMIA; BINDING; SIRNA;

D O I：

10.1016/j.febslet.2010.09.031

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Double-stranded RNAs suppress the expression of homologous genes through an evolutionarily conserved process called RNA interference (RNAi) or post-transcriptional gene silencing. A bidentate nuclease called Dicer has been implicated as the protein responsible for the production of short interfering RNAs (siRNAs). In our experiments, Rex overexpression reduced the efficiency of short hairpin RNA (shRNA)-mediated RNAi. The interaction of Dicer with Rex inhibited the conversion of shRNA to siRNA. These results suggest that the interaction of Dicer with HTLV-I Rex inhibits Dicer activity and thereby reduces the efficiency of the conversion of shRNA to siRNA. Structured summary: MINT-7996954: Rex (uniprotkb:P0C205) physically interacts (MI:0915) with Dicer (uniprotkb:Q9UPY3) by pull down (MI:0096) MINT-7996926: Rex (uniprotkb:P0C205) and Dicer (uniprotkb:Q9UPY3) colocalize (MI:0403) by fluorescence microscopy (MI:0416) MINT-7996905: Dicer (uniprotkb:Q9UPY3) physically interacts (MI:0915) with Rex (uniprotkb:P0C205) by anti bait coimmunoprecipitation (MI:0006) MINT-7996852, MINT-7996870, MINT-7996886, MINT-7996830: Rex (uniprotkb:P0C205) physically interacts (MI:0915) with Dicer (uniprotkb:Q9UPY3) by anti tag coimmunoprecipitation (MI:0007) (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：4313 / 4318

页数：6

共 36 条

[1] FUNCTIONAL-ANALYSIS OF HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I REX-RESPONSE ELEMENT - DIRECT RNA-BINDING OF REX PROTEIN CORRELATES WITH INVIVO ACTIVITY [J].

BALLAUN, C ;

FARRINGTON, GK ;

DOBROVNIK, M ;

RUSCHE, J ;

HAUBER, J ;

BOHNLEIN, E .

JOURNAL OF VIROLOGY, 1991, 65 (08) :4408-4413

[2] Evidence that HIV-1 encodes an siRNA and a suppressor of RNA silencing [J].

Bennasser, Y ;

Le, SY ;

Benkirane, M ;

Jeang, KT .

IMMUNITY, 2005, 22 (05) :607-619

[3] HIV-I tat interaction with Dicer: requirement for RNA [J].

Bennasser, Yamina ;

Jeang, Kuan-Teh .

RETROVIROLOGY, 2006, 3 (1)

[4] Role for a bidentate ribonuclease in the initiation step of RNA interference [J].

Bernstein, E ;

Caudy, AA ;

Hammond, SM ;

Hannon, GJ .

NATURE, 2001, 409 (6818) :363-366

[5]

Bogerd HP, 1996, MOL CELL BIOL, V16, P4207

[6] THE TYPE-I HUMAN T-CELL LEUKEMIA-VIRUS (HTLV-I) REX TRANSACTIVATOR BINDS DIRECTLY TO THE HTLV-I REX AND THE TYPE-1 HUMAN-IMMUNODEFICIENCY-VIRUS REV RNA RESPONSE ELEMENTS [J].

BOGERD, HP ;

HUCKABY, GL ;

AHMED, YF ;

HANLY, SM ;

GREENE, WC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (13) :5704-5708

[7] The HTLV-1 Tax interactome [J].

Boxus, Mathieu ;

Twizere, Jean-Claude ;

Legros, Sebastien ;

Dewulf, Jean-Francois ;

Kettmann, Richard ;

Willems, Luc .

RETROVIROLOGY, 2008, 5 (1)

[8] The influenza A virus NS1 protein binds small interfering RNAs and suppresses RNA silencing in plants [J].

Bucher, E ;

Hemmes, H ;

de Haan, P ;

Goldbach, R ;

Prins, M .

JOURNAL OF GENERAL VIROLOGY, 2004, 85 :983-991

[9] HCV core protein interacts with Dicer to antagonize RNA silencing [J].

Chen, Weixian ;

Zhang, Zhenzhen ;

Chen, Juan ;

Zhang, Juan ;

Zhang, Jun ;

Wu, Ying ;

Huang, Ying ;

Cai, Xuefei ;

Huang, Ailong .

VIRUS RESEARCH, 2008, 133 (02) :250-258

[10] MECHANISM OF ACTION OF REGULATORY PROTEINS ENCODED BY COMPLEX RETROVIRUSES [J].

CULLEN, BR .

MICROBIOLOGICAL REVIEWS, 1992, 56 (03) :375-394

← 1 2 3 4 →