DNA microarray analysis of cyanobacterial gene expression during acclimation to high light

被引:363
作者
Hihara, Y [1 ]
Kamei, A
Kanehisa, M
Kaplan, A
Ikeuchi, M
机构
[1] Saitama Univ, Fac Sci, Dept Biochem & Mol Biol, Urawa, Saitama 3388570, Japan
[2] Univ Tokyo, Grad Sch Arts & Sci, Dept Life Sci, Tokyo 1538902, Japan
[3] Kyoto Univ, Inst Chem Res, Kyoto 6110011, Japan
[4] Hebrew Univ Jerusalem, Dept Plant Sci, IL-91904 Jerusalem, Israel
关键词
D O I
10.1105/tpc.13.4.793
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
DNA microarrays bearing nearly all of the genes of the unicellular cyanobacterium Synechocystis sp PCC 6803 were used to examine the temporal program of gene expression during acclimation from low to high light intensity. A complete pattern is provided of gene expression during acclimation of a photosynthetic organism to changing light intensity, More than 160 responsive genes were identified and classified into distinct sets. Genes involved in light absorption and photochemical reactions were downregulated within 15 min of exposure to high light intensity, whereas those associated with CO2 fixation and protection from photoinhibition were upregulated. Changes in the expression of genes involved in replication, transcription, and translation, which were induced to support cellular proliferation, occurred later. Several unidentified open reading frames were induced or repressed, The possible involvement of these genes in the acclimation to high light conditions is discussed.
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页码:793 / 806
页数:14
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