Long range surface plasmons for observation of biomolecular binding events at metallic surfaces

被引:138
作者
Dostalek, Jakub [1 ]
Kasry, Amal [1 ]
Knoll, Wolfgang [1 ]
机构
[1] Max Planck Inst Polymer Res, Dept Mat Sci, D-55128 Mainz, Germany
关键词
surface plasmon resonance; long range surface plasmon; biosensor; fluorescence spectroscopy; optical sensor;
D O I
10.1007/s11468-007-9037-8
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A long range surface plasmon (LRSP) is an electromagnetic wave propagating along a thin metal film with an order of magnitude lower damping than conventional surface plasmon (SP) waves. Thus, the excitation of LRSP is associated with a narrower resonance and it provides larger enhancement of intensity of the electromagnetic field. In surface plasmon resonance (SPR) biosensors, these features allow a more precise observation of the binding of biomolecules in the proximity to the metal surface by using the (label-free) measurement of refractive index (RI) variations and by SP-enhanced fluorescence spectroscopy. In this contribution, we investigate LRSPs excited on a layer structure consisting of a fluoropolymer buffer layer, a thin gold film, and an aqueous sample. By implementing such structure in an SPR sensor, we achieved a 2.4- and 4.4-fold improvement of the resolution in the label-free and fluorescence-based detection, respectively, of the binding of biomolecules in the close proximity to the surface. Moreover, we demonstrate that the sensor resolution can be improved by a factor of 14 and 12 for the label-free and fluorescence-based detection, respectively, if the biomolecular binding events occur within the whole evanescent field of LRSP.
引用
收藏
页码:97 / 106
页数:10
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