High-performance liquid chromatographic determination and standardization of agmatine

被引：14

作者：

Bencsik, K

Kremmer, T

Boldizár, M

Tamás, J

Mák, M

Páldi, E

机构：

[1] Natl Inst Oncol, Dept Biochem, H-1525 Budapest, Hungary

[2] Hungarian Acad Sci, Cent Res Inst Chem, H-1525 Budapest, Hungary

[3] Hungarian Acad Sci, Agr Res Inst, H-2462 Martonvasar, Hungary

来源：

JOURNAL OF CHROMATOGRAPHY A | 1998年 / 824卷 / 02期

关键词：

agmatine; polyamines;

D O I：

10.1016/S0021-9673(97)00193-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to standardize the reversed-phase high-performance liquid chromatographic (RP-HPLC) determination of natural polyamines, particularly of agmatine, which is important in plant physiology, a simple dansylation and thin-layer chromatography (TLC) procedure was developed for the separation and purification of dansyl-agmatine. The monodansyl structure of the agmatine derivative was verified by mass spectrometry. Dansyl-agmatine was used as a reliable reference compound for the validation of an improved RP-HPLC method and to the quantitation of plant polyamines. In two varieties of wheat that had been stressed by cold treatment, significant changes were demonstrated in the agmatine content of plant seedlings. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：175 / 180

页数：6

共 15 条

[1] RP-HPLC SEPARATION OF POLYAMINES AFTER AUTOMATIC FMOC-CI DERIVATIZATION AND PRECOLUMN SAMPLE CLEANUP USING COLUMN SWITCHING [J].