Micro-Raman spectroscopy detects individual neoplastic and normal hematopoietic cells

被引:570
作者
Chan, JW
Taylor, DS
Zwerdling, T
Lane, SM
Ihara, K
Huser, T
机构
[1] Lawrence Livermore Natl Lab, Livermore, CA 94551 USA
[2] Univ Calif Davis, Med Ctr, Dept Pediat, Hematol Oncol Sect, Sacramento, CA 95817 USA
[3] Univ Calif Davis, NSF, Ctr Biophoton Sci & Technol, Sacramento, CA 95817 USA
基金
美国国家科学基金会;
关键词
D O I
10.1529/biophysj.105.066761
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Current methods for identifying neoplastic cells and discerning them from their normal counterparts are often nonspecific, slow, biologically perturbing, or a combination thereof. Here, we show that single-cell micro-Raman spectroscopy averts these shortcomings and can be used to discriminate between unfixed normal human lymphocytes and transformed Jurkat and Raji lymphocyte cell lines based on their biomolecular Raman signatures. We demonstrate that single-cell Raman spectra provide a highly reproducible biomolecular fingerprint of each cell type. Characteristic peaks, mostly due to different DNA and protein concentrations, allow for discerning normal lymphocytes from transformed lymphocytes with high confidence (p << 0.05). Spectra are also compared and analyzed by principal component analysis to demonstrate that normal and transformed cells form distinct clusters that can be defined using just two principal components. The method is shown to have a sensitivity of 98.3% for cancer detection, with 97.2% of the cells being correctly classified as belonging to the normal or transformed type. These results demonstrate the potential application of confocal micro-Raman spectroscopy as a clinical tool for single cancer cell detection based on intrinsic biomolecular signatures, therefore eliminating the need for exogenous fluorescent labeling.
引用
收藏
页码:648 / 656
页数:9
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