Crystallization of a large single crystal of a B-DNA decamer for a neutron diffraction experiment by the phase-diagram technique

被引：21

作者：

Arai, S ^{[1
]}

Chatake, T ^{[1
]}

Minezaki, Y ^{[1
]}

Niimura, N ^{[1
]}

机构：

[1] Japan Atom Energy Res Inst, Adv Sci Res Ctr, Tokai, Ibaraki 3191195, Japan

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | 2002年 / 58卷

关键词：

D O I：

10.1107/S0907444901017401

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Crystallization of a large single crystal of a B-DNA decamer, d(CCATTAATGG), for a neutron-diffraction experiment has been accomplished by an analysis of its solubility phase diagram and a large single crystal was successfully crystallized at around the minimum solubility point of the oligonucleotide: 30%(v/v) MPD, 100 mM MgCl2 pD 6.6 using 0.4 ml D2O solutions of the DNA (sample concentration 1.5 mM). It is confirmed that the resulting crystal (dimensions: 1.7 x 1.3 x 0.6 mm) diffracts sufficiently well for neutron data collection.

引用

页码：151 / 153

页数：3

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