Use of LC-MS/MS to cross-validate a radioimmunoassay for the fibrinogen receptor antagonist, Aggrastat (tirofiban hydrochloride) in human plasma

被引：14

作者：

Ellis, JD ^{[1
]}

Hand, EL ^{[1
]}

Gilbert, JD ^{[1
]}

机构：

[1] MERCK RES LABS,DEPT DRUG METAB 1,W POINT,PA 19486

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1997年 / 15卷 / 05期

关键词：

Aggrastat; tirofiban hydrochloride; plasma; radioimmunoassay; liquid chromatography tandem mass spectrometry; cross-validation;

D O I：

10.1016/S0731-7085(96)01848-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A method based on LC-MS/MS was developed for the determination of the fibrinogen-receptor antagonist Aggrastat in human plasma. The drug is isolated from plasma by liquid extraction and converted into its N-trifluoroacetyl derivative prior to analysis by HPLC with atmospheric pressure negative chemical ionization MS/MS detection. A structural analog is used as the internal standard and the lower quantifiable limit of the assay is 0.4 ng ml(-1) with a relative standard deviation of 7%. This assay was used to cross-validate the existing immunoassay by analysis of plasma from patients receiving the drug. The specificity of the immunoassay was thereby confirmed.

引用

收藏

页码：561 / 569

页数：9

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