Sequencing Analysis of the Medicinal Plant Stemona tuberosa and Five Related Species Existing in Thailand Based on trnH-psbA Chloroplast DNA

被引：17

作者：

Vongsak, Boonyadist ^{[1
]}

Kengtong, Surapong ^{[2
]}

Vajrodaya, Srunya ^{[3
]}

Sukrong, Suchada ^{[1
]}

机构：

[1] Chulalongkorn Univ, Fac Pharmaceut Sci, CU Drugs & Hlth Prod Innovat & Promot Ctr, Dept Pharmacognosy, Bangkok 10330, Thailand

[2] Chulalongkorn Univ, Dept Pharmaceut Bot, Bangkok 10330, Thailand

[3] Kasetsart Univ, Fac Sci, Dept Bot, Bangkok, Thailand

来源：

PLANTA MEDICA | 2008年 / 74卷 / 14期

关键词：

Stemonaceae; Stemona tuberosa; trnH-psbA; sequence analysis; chloroplast DNA;

D O I：

10.1055/s-0028-1088308

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

In Thailand, Stemona tuberosa Lour., S.phyllantha Gagnep., S. collinscae Craib, S. burkillii Prain, S. aphylla Craib and S. sp. are found. The identification based oil morphological characters alone is difficult and can lead to confusion regarding chemical constituents and biological activities. The tuberous roots Of S. tuberosa have long been used for treatment of respiratory diseases and as anthelmintics. However, accurate identification of S. tuberosa is needed to ensure efficacy. Sequence comparison indicated that these Stemona spp. could be identified from the sequence of the trnH-psbA locus. As a result of different sequence lengths, the PCR products generated from newly designed primers could be used to preliminarily group the two species, S. tuberosa and S. phyllantha, apart from others. However, these products Could be further sequenced to discriminate among Stemona spp.

引用

页码：1764 / 1766

页数：3

共 8 条

[1]

Chung HS, 2003, PLANTA MED, V69, P914, DOI 10.1055/s-2003-45100

[2] Structural relationships, distribution and biological activities of Stemona alkaloids [J].