Dual-color 4Pi-confocal microscopy with 3D-resolution in the 100 nm range

被引：25

作者：

Kano, H ^{[1
]}

Jakobs, S ^{[1
]}

Nagorni, M ^{[1
]}

Hell, SW ^{[1
]}

机构：

[1] Max Planck Inst Biophys Chem, High Resolut Opt Microscopy Grp, D-37070 Gottingen, Germany

来源：

ULTRAMICROSCOPY | 2002年 / 90卷 / 2-3期

关键词：

D O I：

10.1016/S0304-3991(01)00132-2

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We report the development of simultaneous two-color channel recording in 4Pi-confocal microscopy. A marked increase of spatial resolution over confocal microscopy becomes manifested in 4Pi-confocal three-dimensional (313) data stacks of dual-labeled objects. The fundamentally improved resolution is verified both with densely labeled fluorescence beads as well as with membrane labeled fixed Escherichia coli. The synergistic combination of dual-color 4Pi-confocal recording with image restoration results in dual-color imaging with a 3D resolution in the 100 nm range. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：207 / 213

页数：7

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