Chromatographic purification of biliproteins from Spirulina platensis - High-performance liquid chromatographic separation of their alpha and beta subunits

被引：56

作者：

Bermejo, R ^{[1
]}

Talavera, EM ^{[1
]}

AlvarezPez, JM ^{[1
]}

Orte, JC ^{[1
]}

机构：

[1] UNIV GRANADA, DEPT CHEM PHYS, E-18071 GRANADA, SPAIN

来源：

JOURNAL OF CHROMATOGRAPHY A | 1997年 / 778卷 / 1-2期

关键词：

Spirulina platensis; biliproteins; proteins; phycobilisomes;

D O I：

10.1016/S0021-9673(97)00577-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A fast preparative two-step chromatographic method for purification of C-phycocyanin and allophycocyanin from Spirulina platensis is described. Both biliproteins were homogeneous as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis yielding two closely migrating bands. Separation of alpha and beta subunits from C-phycocyanin and allophycocyanin was also accomplished by a chromatographic procedure under denaturing conditions. The phycocyanobilin chromophore from both biliproteins has been isolated, tested for purity by reversed-phase high performance liquid chromatography and spectroscopically characterized. (C) 1997 Elsevier Science B.V.

引用

页码：441 / 450

页数：10

共 27 条

[1]

[Anonymous], 1982, BIOL CYANOBACTERIA

[2]

BERMEJO R, 1994, THESIS U GRANADA GRA

[3]

ISOLATION AND CHARACTERIZATION OF PHYCOCYANINS FROM THE BLUE-GREEN-ALGA SPIRULINA-PLATENSIS [J].