Fatty acid alteration and the lateral diffusion of lipids in the plasma membrane of keratinocytes

被引:17
作者
Fulbright, RM
Axelrod, D
Dunham, WR
Marcelo, CL
机构
[1] UNIV MICHIGAN,DIV BIOPHYS RES,ANN ARBOR,MI 48109
[2] UNIV MICHIGAN,DEPT PHYS,ANN ARBOR,MI 48109
[3] UNIV MICHIGAN,SCH MED,DEPT SURG,DIV PLAST & RECONSTRUCT SURG,ANN ARBOR,MI 48109
关键词
D O I
10.1006/excr.1997.3553
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
The fluorescent probe diI was used to study the lateral mobility of lipids in in vitro strains of Living adult human keratinocytes grown in four different media, One medium was essential fatty acid deficient (EFAD) and low in calcium ion, a medium known to yield cells that proliferate rapidly and contain lipid with extremely low levels of essential fatty acids. Two other media were supplemented with essential fatty acids (FAS), media that are known to result in cells that grow more slowly and have normalized fatty acid proportions, A fourth medium consisted of 1 mu M all-trans-retinoic acid added to the fatty acid-supplemented medium (FAS-RA), a medium known to produce cells that are highly proliferative, with a growth rate greater than that of the FAS strains and similar to that of the EFAD strains, The keratinocytes grown in these four media were studied using the fluorescence recovery after photobleaching (FRAP) technique to determine the lateral diffusion rate of diI in the plasma membranes, Our results showed a positive correlation between growth rate and diffusion coefficient (D): the diffusion coefficient of diI was higher in the EFAD or FAS-RA cells than in the FAS cells. The measurement of D among the FAS cells fell into two groups, One group was similar to the single group seen in the EFAD cells, but the other group was composed of much lower D values. The other FRAP parameters (mobile fraction and bleach depth) were larger in the ''slow'' group than in the ''fast'' group, This trend of negative correlation between these parameters and D was also found within the fast group. These results are interpreted in terms of possible changes in membrane structure or morphology that might be indirectly associated with the fatty acid alterations, including the possible presence of areas in senescing keratinocytes where plasma membranes collapse to form an interacting system of lipid bilayers. (C) 1997 Academic Press.
引用
收藏
页码:128 / 134
页数:7
相关论文
共 30 条
[1]   THE EXPRESSION OF MUTANT EPIDERMAL KERATIN CDNAS TRANSFECTED IN SIMPLE EPITHELIAL AND SQUAMOUS-CELL CARCINOMA LINES [J].
ALBERS, K ;
FUCHS, E .
JOURNAL OF CELL BIOLOGY, 1987, 105 (02) :791-806
[2]   EXPRESSION OF MUTANT KERATIN CDNAS IN EPITHELIAL-CELLS REVEALS POSSIBLE MECHANISMS FOR INITIATION AND ASSEMBLY OF INTERMEDIATE FILAMENTS [J].
ALBERS, K ;
FUCHS, E .
JOURNAL OF CELL BIOLOGY, 1989, 108 (04) :1477-1493
[3]   MOBILITY MEASUREMENT BY ANALYSIS OF FLUORESCENCE PHOTOBLEACHING RECOVERY KINETICS [J].
AXELROD, D ;
KOPPEL, DE ;
SCHLESSINGER, J ;
ELSON, E ;
WEBB, WW .
BIOPHYSICAL JOURNAL, 1976, 16 (09) :1055-1069
[4]   CARBOCYANINE DYE ORIENTATION IN RED-CELL MEMBRANE STUDIED BY MICROSCOPIC FLUORESCENCE POLARIZATION [J].
AXELROD, D .
BIOPHYSICAL JOURNAL, 1979, 26 (03) :557-573
[5]   DIGITAL IMAGING FLUORESCENCE MICROSCOPY - SPATIAL HETEROGENEITY OF PHOTOBLEACHING RATE CONSTANTS IN INDIVIDUAL CELLS [J].
BENSON, DM ;
BRYAN, J ;
PLANT, AL ;
GOTTO, AM ;
SMITH, LC .
JOURNAL OF CELL BIOLOGY, 1985, 100 (04) :1309-1323
[6]  
Bevington R., 1969, DATA REDUCTION ERROR
[7]   DELETIONS IN EPIDERMAL KERATINS LEADING TO ALTERATIONS IN FILAMENT ORGANIZATION INVIVO AND IN INTERMEDIATE FILAMENT ASSEMBLY INVITRO [J].
COULOMBE, PA ;
CHAN, YM ;
ALBERS, K ;
FUCHS, E .
JOURNAL OF CELL BIOLOGY, 1990, 111 (06) :3049-3064
[8]   EPR measurements showing that plasma membrane viscosity can vary from 30 to 100 cP in human epidermal cell strains [J].
Dunham, WR ;
Sands, RH ;
Klein, SB ;
Duell, EA ;
Rhodes, LM ;
Marcelo, CL .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 1996, 52 (10) :1357-1368
[9]   THE EFFECT OF ESSENTIAL FATTY-ACID SUPPLEMENTATION ON KERATINOCYTE REPLICATION [J].
GARNER, WL ;
OYATSU, Y ;
ZUCCARO, C ;
RODRIQUEZ, JL ;
SMITH, DJ ;
MARCELO, CL .
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1995, 52 (05) :349-355
[10]   MONOPALMITOYLPHOSPHATIDYLCHOLINE INCORPORATION INTO HUMAN-ERYTHROCYTE GHOST MEMBRANES CAUSES PROTEIN AND LIPID IMMOBILIZATION AND CHOLESTEROL DEPLETION [J].
GOLAN, DE ;
FURLONG, ST ;
BROWN, CS ;
CAULFIELD, JP .
BIOCHEMISTRY, 1988, 27 (08) :2661-2667