High-throughput screening for modulators of stem cell differentiation

被引：23

作者：

Bushway, Paul J. ^{[1
]}

Mercola, Mark ^{[1
]}

机构：

[1] Burke Med Res Inst, La Jolla, CA USA

来源：

MEASURING BIOLOGICAL RESPONSES WITH AUTOMATED MICROSCOPY | 2006年 / 414卷

关键词：

D O I：

10.1016/S0076-6879(06)14017-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Realizing the potential of stem cell biology requires the modulation of self-renewal and differentiation, both of which are incompletely understood. This chapter describes methods for the design, development, and implementation of cell-based screens of small molecules, genes and expressed proteins for modulation of stem and progenitor cell fate. These include the engineering of embryonic and other stem cells with gene promoter-reporter protein constructs and their application in automated screening. We discuss considerations of promoter reporter selection, assay development and implementation, and image acquisition, analysis, and data handling. Such black-box screens are useful for the identification of probes of developmental processes and should provide tools that will identify druggable targets for biochemical assays.

引用

收藏

页码：300 / 316

页数：17

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