XAS of dilute biological samples

被引：8

作者：

Ranieri-Raggi, M

Raggi, A

Martini, D

Benvenuti, M

Mangani, S

机构：

[1] Univ Siena, Dept Chem, I-53100 Siena, Italy

[2] Univ Pisa, Dept Environm & Human Sci, I-56126 Pisa, Italy

来源：

JOURNAL OF SYNCHROTRON RADIATION | 2003年 / 10卷

关键词：

X-ray absorption spectroscopy (XAS); biological systems;

D O I：

10.1107/S0909049502017260

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The experimental setup of beamline ID26 at ESRF (Grenoble) has been successfully exploited to obtain high-quality XAS (X-ray absorption spectroscopy) data from a biological sample where the metal concentration is about 100 muM. The sample consists of the adenosine monophosphate deaminase (AMPD) histidine proline rich glycoprotein (HPRG) complex that contains 3-4 Zn(II) ions per dimer of approximately 320 kDa molecular weight. The experiment shows that third-generation X-ray sources equipped with insertion devices and appropriate optics and detectors allow the investigation of complex biological systems where the metal concentration is intrinsically low. The availability of such experimental setups makes possible a completely new set of experiments in biological XAS.

引用

页码：69 / 70

页数：2

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