Rapid staining and enumeration of small numbers of total bacteria in water by solid-phase laser cytometry

被引:16
作者
Broadaway, SC [1 ]
Barton, SA [1 ]
Pyle, BH [1 ]
机构
[1] Montana State Univ, Dept Microbiol, Bozeman, MT 59717 USA
关键词
D O I
10.1128/AEM.69.7.4272-4273.2003
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The nucleic acid stain SYBR Green I was evaluated for use with solid-phase laser cytometry to obtain total bacterial cell counts from several water sources with small bacterial numbers. Results were obtained within 30 min and exceeded or equaled counts on R2A agar plates incubated for 14 days at room temperature.
引用
收藏
页码:4272 / 4273
页数:2
相关论文
共 14 条
[1]  
Barbesti S, 2000, CYTOMETRY, V40, P214, DOI 10.1002/1097-0320(20000701)40:3<214::AID-CYTO6>3.0.CO
[2]  
2-M
[3]  
Daley R. J., 1979, ASTM STP, P29
[4]  
GRIGOROVA R, 1990, METHDOS MICROBIOLOGY, V22, P8
[5]  
JONES DL, 1999, PHARMACOPEIAL FORUM, V25, P7626
[6]   Use of fluorescent probes to assess physiological functions of bacteria at single-cell level [J].
Joux, F ;
Lebaron, P .
MICROBES AND INFECTION, 2000, 2 (12) :1523-1535
[7]   USE OF FLUOROCHROMES FOR DIRECT ENUMERATION OF TOTAL BACTERIA IN ENVIRONMENTAL-SAMPLES - PAST AND PRESENT [J].
KEPNER, RL ;
PRATT, JR .
MICROBIOLOGICAL REVIEWS, 1994, 58 (04) :603-615
[8]  
Lebaron P, 1998, APPL ENVIRON MICROB, V64, P1725
[9]   Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometry using the nucleic acid stain SYBR Green I [J].
Marie, D ;
Partensky, F ;
Jacquet, S ;
Vaulot, D .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (01) :186-193
[10]  
MignonGodefroy K, 1997, CYTOMETRY, V27, P336