WIN55,212-2-mediated inhibition of HIV-1 expression in microglial cells: Involvement of cannabinoid receptors

被引：47

作者：

Rock, R. Bryan

Gekker, Genya

Hu, Shuxian

Sheng, Wen S.

Cabral, Guy A.

Martin, Billy R.

Peterson, Phillip K.

机构：

[1] Univ Minnesota, Dept Med, Div Infect Dis & Int Med, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Sch Med, Ctr Infect Dis & Microbiol Translat Res, Minneapolis, MN 55455 USA

[3] Univ Minnesota, Sch Med, Dept Med, Minneapolis, MN 55455 USA

[4] Virginia Commonwealth Univ, Dept Microbiol & Immunol, Richmond, VA 23298 USA

[5] Virginia Commonwealth Univ, Dept Pharmacol & Toxicol, Richmond, VA 23298 USA

来源：

JOURNAL OF NEUROIMMUNE PHARMACOLOGY | 2007年 / 2卷 / 02期

关键词：

cannabinoids; HIV-1; microglia; WIN55,212-2; SR141716A;

D O I：

10.1007/s11481-006-9040-4

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Cannabinoid receptors CB1 and CB2 are primarily expressed in cells of the nervous and immune systems, respectively. Recently, the synthetic CB1/CB2 agonist WIN55,212-2 was found to suppress replication of HIV-1 in microglial cell cultures. The present study was undertaken to test the hypothesis that WIN 55,212-2's antiviral effect is mediated via CB2 receptors. By reverse transcription-polymerase chain reaction, microglia were found to express both CB, and CB2 receptors. Using additional CBI/ CB2 receptor agonists and selective antagonists, we found that CB2 receptors are involved in WIN55,212-2's antiviral activity and surprisingly that the CB, receptor-selective antagonist SR141716A behaved as an agonist in these brain macrophages.

引用

页码：178 / 183

页数：6

共 20 条

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