The biology of prothrombin

被引:23
作者
Degen, SJF
Sun, WY
机构
[1] Childrens Hosp Res Fdn, Dept Pediat, Div Dev Biol, Cincinnati, OH 45229 USA
[2] Univ Cincinnati, Cincinnati, OH USA
来源
CRITICAL REVIEWS IN EUKARYOTIC GENE EXPRESSION | 1998年 / 8卷 / 02期
关键词
blood coagulation; kringle domains; vitamin K-dependent protein; gene targeting; prothrombin deficiency; HNF-1;
D O I
10.1615/CritRevEukarGeneExpr.v8.i2.60
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Prothrombin and thrombin are involved in diverse biological functions. The structure of prothrombin has been studied extensively and its cDNA has been cloned from several species. The tissue-specific expression of this protein has been studied, as well as the developmental expression pattern. The structure of the human gene coding for prothrombin has been determined, and gene regulation studies have been performed that indicate that HNF-1 might be responsible for the liver-specific expression of this protein. Other regulatory elements have been identified. In order to further study the biological properties of prothrombin, prothrombin-deficient mice have been generated using gene targeting technology. Prothrombin deficiency in mice results in partial embryonic lethality. The mice that survive to birth die from bleeding events. The embryonic lethality occurs between embryonic days 9.5 and 11.5 and appears to be due to the loss of integrity of the vasculature due to a failure in blood coagulation. These results indicate that prothrombin plays not only a key role in hemostasis but suggests that it may be important for mouse development.
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页码:203 / 224
页数:22
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