Behind the scenes of a small RNA gene-silencing pathway

被引：17

作者：

Ku, Gregory ^{[1
,2
]}

McManus, Michael T. ^{[1
,3
]}

机构：

[1] Univ Calif San Francisco, UCSF Diabet Ctr, San Francisco, CA 94122 USA

[2] Univ Calif San Francisco, Dept Med, Div Endocrinol & Metab, San Francisco, CA 94122 USA

[3] Univ Calif San Francisco, Dept Microbiol & Immunol, San Francisco, CA 94122 USA

来源：

HUMAN GENE THERAPY | 2008年 / 19卷 / 01期

关键词：

D O I：

10.1089/hum.2007.1226

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

RNA interference (RNAi) is a sequence-specific gene-silencing phenomenon mediated by double-stranded RNA. RNAi is made possible through the activity of an evolutionarily conserved RNA-protein machinery that is utilized by microRNAs (miRNAs), endogenously produced 21- to 23-nucleotide noncoding RNAs. As RNA-based therapeutics come of age, understanding the miRNA pathway and the mechanism of posttranscriptional gene silencing is paramount. Studies have pointed to a critical role for miRNAs in human development and disease and revealed an unanticipated complexity in the mechanisms of regulation of gene expression. This review discusses advances in the biogenesis of miRNAs, their modes of action, and implications for RNA- based treatments.

引用

页码：17 / 26

页数：10

共 119 条

[91] Identification of mammalian microRNA host genes and transcription units [J].

Rodriguez, A ;

Griffiths-Jones, S ;

Ashurst, JL ;

Bradley, A .

GENOME RESEARCH, 2004, 14 (10A) :1902-1910

[92] Requirement of bic/microRNA-155 for normal immune function [J].

Rodriguez, Antony ;

Vigorito, Elena ;

Clare, Simon ;

Warren, Madhuri V. ;

Couttet, Philippe ;

Soond, Dalya R. ;

van Dongen, Stijn ;

Grocock, Russell J. ;

Das, Partha P. ;

Miska, Eric A. ;

Vetrie, David ;

Okkenhaug, Klaus ;

Enright, Anton J. ;

Dougan, Gordon ;

Turner, Martin ;

Bradley, Allan .

SCIENCE, 2007, 316 (5824) :608-611

[93] Intronic microRNA precursors that bypass Drosha processing [J].

Ruby, J. Graham ;

Jan, Calvin H. ;

Bartel, David P. .

NATURE, 2007, 448 (7149) :83-86

[94] Asymmetry in the assembly of the RNAi enzyme complex (Reprinted from Cell, vol 115, pg 199-208, 2003) [J].

Schwarz, Dianne S. ;

Hutvagner, Gyoergy ;

Du, Tingting ;

Xu, Zuoshang ;

Aronin, Neil ;

Zamore, Phillip D. .

CELL, 2007, 131 (04) :30-40

[95] Two genetic circuits repress the Caenorhabditis elegans heterochronic gene lin-28 after translation initiation [J].

Seggerson, K ;

Tang, LJ ;

Moss, EG .

DEVELOPMENTAL BIOLOGY, 2002, 243 (02) :215-225

[96] A guide through present computational approaches for the identification of mammalian microRNA targets [J].

Sethupathy, Praveen ;

Megraw, Molly ;

Hatzigeorgiou, Artemis G. .

NATURE METHODS, 2006, 3 (11) :881-886

[97] Uridine addition after microRNA-directed cleavage [J].

Shen, BZ ;

Goodman, HM .

SCIENCE, 2004, 306 (5698) :997-997

[98] A DNA vector-based RNAi technology to suppress gene expression in mammalian cells [J].

Sui, GC ;

Soohoo, C ;

Affar, E ;

Gay, F ;

Shi, YJ ;

Forrester, WC ;

Shi, Y .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (08) :5515-5520

[99] Novel and stress-regulated microRNAs and other small RNAs from Arabidopsis [J].

Sunkar, R ;

Zhu, JK .

PLANT CELL, 2004, 16 (08) :2001-2019

[100] NF-κB-dependent induction of microRNA miR-146, an inhibitor targeted to signaling proteins of innate immune responses [J].

Taganov, Konstantin D. ;

Boldin, Mark P. ;

Chang, Kuang-Jung ;

Baltimore, David .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (33) :12481-12486

← 3 4 5 6 7 8 9 10 11 12 →