Biomass estimation of Aspergillus niger growing on real and model supports in solid state fermentation

被引：34

作者：

CordovaLopez, J ^{[1
]}

GutierrezRojas, M ^{[1
]}

Huerta, S ^{[1
]}

SaucedoCastaneda, G ^{[1
]}

FavelaTorres, E ^{[1
]}

机构：

[1] UNIV AUTONOMA METROPOLITANA IZTAPALAPA,DEPT BIOTECNOL,MEXICO CITY 09340,DF,MEXICO

来源：

BIOTECHNOLOGY TECHNIQUES | 1996年 / 10卷 / 01期

关键词：

D O I：

10.1007/BF00161075

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Direct hydrolysis of Aspergillus niger mycelium growth on amberlite IRA-900 or sugar cane bagasse on solid state fermentation followed by the analysis of soluble protein by the dye binding method was carried out. Hydrolysis with phosphoric acid 0.25M during 7 min allowed maxima protein extraction available to be measured. Color interference of medium components was not observed, allowing the use of this method for biomass estimation when amberlite IRA-900 or sugar cane bagasse are used as support in solid state fermentation processes.

引用

页码：1 / 6

页数：6

共 13 条

[1]

AURIA R, 1990, BIOTECHNOL TECH, V4, P391

[2]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[3]

CHANG LT, 1986, MANUAL IND MICROBIOL

[4] MECHANISM OF DYE RESPONSE AND INTERFERENCE IN THE BRADFORD PROTEIN ASSAY [J].

COMPTON, SJ ;

JONES, CG .

ANALYTICAL BIOCHEMISTRY, 1985, 151 (02) :369-374

[5]

Corpe W. A., 1980, ADSORPTION MICROORGA, P105

[6]

DESGRANGES C, 1991, APPL MICROBIOL BIOT, V35, P200

[7] CITRIC-ACID AND POLYOLS PRODUCTION BY ASPERGILLUS-NIGER AT HIGH GLUCOSE-CONCENTRATION IN SOLID-STATE FERMENTATION ON INERT SUPPORT [J].

GUTIERREZROJAS, M ;

CORDOVA, J ;

AURIA, R ;

REVAH, S ;

FAVELATORRES, E .

BIOTECHNOLOGY LETTERS, 1995, 17 (02) :219-224

[8] COMPARISON OF DIFFERENT METHODS FOR PROTEIN DETERMINATION IN ASPERGILLUS-NIGER MYCELIUM [J].

JERNEJC, K ;

CIMERMAN, A ;

PERDIH, A .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1986, 23 (06) :445-448

[9] STERIC HINDRANCE OF GROWTH OF FILAMENTOUS FUNGI IN SOLID SUBSTRATE FERMENTATION OF WHEAT STRAW [J].

LAUKEVICS, JJ ;

APSITE, AF ;

VIESTURS, US ;

TENGERDY, RP .

BIOTECHNOLOGY AND BIOENGINEERING, 1985, 27 (12) :1687-1691

[10] SOLID-STATE CULTURE OF ASPERGILLUS-NIGER ON SUPPORT [J].

ORIOL, E ;

SCHETTINO, B ;

VINIEGRAGONZALES, G ;

RAIMBAULT, M .

JOURNAL OF FERMENTATION TECHNOLOGY, 1988, 66 (01) :57-62

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