An improved cryogen for plunge freezing

被引：204

作者：

Tivol, William F. ^{[1
]}

Briegel, Ariane ^{[1
]}

Jensen, Grant J. ^{[1
]}

机构：

[1] CALTECH, Broad Ctr, Pasadena, CA 91125 USA

来源：

MICROSCOPY AND MICROANALYSIS | 2008年 / 14卷 / 05期

关键词：

cryogen; plunge freezing; electron cryomicroscopy; ice embedding; transmission electron microscopy; specimen preparation;

D O I：

10.1017/S1431927608080781

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

The use of an alkane mixture that remains liquid at 77 K to freeze specimens has advantages over the use of a pure alkane that is solid at 77 K. It was found that a mixture of methane and ethane did not give a cooling rate adequate to produce vitreous ice, but a mixture of propane and ethane did result in vitreous ice. Furthermore, the latter mixture produced less damage to specimens mounted on a very thin, fragile holey carbon substrate.

引用

页码：375 / 379

页数：5

共 3 条

[1] Location and architecture of the Caulobacter crescentus chemoreceptor array [J].