West Nile virus in Southern Portugal, 2004

被引：57

作者：

Esteves, A

Almeida, APG

Galao, RP

Parreira, R

Piedade, J

Rodrigues, JC

Sousa, CA

Novo, MT

机构：

[1] Univ Nova Lisboa, Inst Higiene & Med Trop, Unidade Virol, P-1200 Lisbon, Portugal

[2] Univ Nova Lisboa, Inst Higiene & Med Trop, Unidade Entomol Med, Unidade Parasitol & Microbiol Med, P-1200 Lisbon, Portugal

来源：

VECTOR-BORNE AND ZOONOTIC DISEASES | 2005年 / 5卷 / 04期

关键词：

Culex mosquitoes; Flavivirus; NS5; gene; Southern Portugal; West Nile virus;

D O I：

10.1089/vbz.2005.5.410

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

West Nile virus (WNV) genomic RNA was detected by reverse transcription-polymerase chain reaction (RT-PCR) in six out of 57 mosquito pools collected in Southern Portugal, during the summer of 2004, yielding an infection rate (IR) of 2.8/1,000 mosquitoes. Phylogenetic analysis of a 217-nucleotide fragment of the NS5 coding region, amplified from Culex pipiens s.l. and Culex univittatus unfed females, demonstrated a close relationship with WNV strains circulating in the Mediterranean basin (Italy, 1998; France, 2000; Morocco, 2003). The data in this short report demonstrate the presence of WNV in mosquitoes in Southern Portugal and the need of permanent surveillance for viral activity within the country.

引用

页码：410 / 413

页数：4

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[4]

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