Structure and expression of the rat mRNA encoding a novel member of the fibroblast growth factor family

被引：263

作者：

Yamasaki, M ^{[1
]}

Miyake, A ^{[1
]}

Tagashira, S ^{[1
]}

Itoh, N ^{[1
]}

机构：

[1] KYOTO UNIV,FAC PHARMACEUT SCI,DEPT BIOCHEM GENET,SAKYO KU,KYOTO 60601,JAPAN

来源：

JOURNAL OF BIOLOGICAL CHEMISTRY | 1996年 / 271卷 / 27期

关键词：

D O I：

10.1074/jbc.271.27.15918

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated the cDNA encoding a novel member of the fibroblast growth factor (FGF) family from rat embryos by homology-based polymerase chain reaction. The FGF-related cDNA encodes a protein of 215 amino acids (similar to 24 kDa), which has a conserved similar to 120 amino acid core with similar to 30-60% amino acid sequence identity with the FGF family, This protein with a hydrophobic amino terminus appears to be a secreted protein. The cDNA was translated in a coupled in vitro transcription-translation system. The molecular mass of the translation product was observed to be similar to 26 kDa. The expression of the FGF-related mRNA in the rat embryo and adult tissues was determined by Northern analysis and in situ hybridization. The mRNA was expressed in several discrete regions of the embryo. In adult tissues, the mRNA was preferentially expressed in the lung. The expression profile of the FGF-related mRNA was different from those of other FGF family mRNAs. As this protein is the 10th documented protein related to FGFs, we tentatively term this protein FGF-10.

引用

页码：15918 / 15921

页数：4

共 15 条

[1] KERATINOCYTE GROWTH-FACTOR - A FIBROBLAST GROWTH-FACTOR FAMILY MEMBER WITH UNUSUAL TARGET-CELL SPECIFICITY [J].

AARONSON, SA ;

BOTTARO, DP ;

MIKI, T ;

RON, D ;

FINCH, PW ;

FLEMING, TP ;

AHN, J ;

TAYLOR, WG ;

RUBIN, JS .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1991, 638 :62-77

[2]

BAIRD A, 1991, CANCER CELL-MON REV, V3, P239

[3] THE HEPARIN-BINDING (FIBROBLAST) GROWTH-FACTOR FAMILY OF PROTEINS [J].

BURGESS, WH ;

MACIAG, T .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :575-606

[4] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[5] THE FGF6 GENE WITHIN THE FGF MULTIGENE FAMILY [J].

COULIER, F ;

OLLENDORFF, V ;

MARICS, I ;

ROSNET, O ;

BATOZ, M ;

PLANCHE, J ;

MARCHETTO, S ;

PEBUSQUE, MJ ;

DELAPEYRIERE, O ;

BIRNBAUM, D .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1991, 638 :53-61

[6] EXPRESSION, PROCESSING, AND PROPERTIES OF INT-2 [J].

DICKSON, C ;

FULLERPACE, F ;

KIEFER, P ;

ACLAND, P ;

MACALLAN, D ;

PETERS, G .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1991, 638 :18-26

[7] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002

[8] EXPRESSION AND POSSIBLE FUNCTIONS OF THE FGF-5 GENE [J].

GOLDFARB, M ;

BATES, B ;

DRUCKER, B ;

HARDIN, J ;

HAUB, O .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1991, 638 :38-52

[9] FGF-7 (KERATINOCYTE GROWTH-FACTOR) EXPRESSION DURING MOUSE DEVELOPMENT SUGGESTS ROLES IN MYOGENESIS, FOREBRAIN REGIONALIZATION AND EPITHELIAL-MESENCHYMAL INTERACTIONS [J].

MASON, IJ ;

FULLERPACE, F ;

SMITH, R ;

DICKSON, C .

MECHANISMS OF DEVELOPMENT, 1994, 45 (01) :15-30

[10] MOLECULAR-CLONING OF A NOVEL CYTOKINE CDNA-ENCODING THE 9TH MEMBER OF THE FIBROBLAST GROWTH-FACTOR FAMILY, WHICH HAS A UNIQUE SECRETION PROPERTY [J].

MIYAMOTO, M ;

NARUO, K ;

SEKO, C ;

MATSUMOTO, S ;

KONDO, T ;

KUROKAWA, T .

MOLECULAR AND CELLULAR BIOLOGY, 1993, 13 (07) :4251-4259

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