Intraspecific polymorphisms of Eimeria species due to resistance against anticoccidial drugs

被引：34

作者：

Greif, G ^{[1
]}

Stephan, B ^{[1
]}

Haberkorn, A ^{[1
]}

机构：

[1] HANNOVER SCH VET MED,INST PARASITOL,D-30559 HANNOVER,GERMANY

来源：

PARASITOLOGY RESEARCH | 1996年 / 82卷 / 08期

关键词：

D O I：

10.1007/s004360050189

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Resistance analyses were done on 15 Eimeria acervulina strains and 5 E. brunetti strains. In all, 55% of these strains proved to have complex profiles of resistance to anticoccidial drugs as judged by resistance-index (RI) evaluation. Genomic fingerprints generated by random amplified DIVA (RAPD) with 16 primers via the polymerase chain reaction (PCR) revealed a high degree of similarity (SI) between nonresistant strains (SI up to 95%). Polymorphisms including band shifts, differences in banding intensity, and missing bands led to significantly low SI values (57%, 69%, 82%) in drug-resistant Eimeria strains. After experimental induction of diclazuril resistance (1, 2, and 4 ppm) in a laboratory isolate VT-1 primer 5'-CCC TGA GAT GGG AAC CTC-3' amplified a polymorphic band of around 600 bp. Polymorphisms detected by RAPD-PCR will facilitate the selection of molecular markers and might lead to the design of diagnostic tests for drug-resistant genotypes.

引用

页码：706 / 714

页数：9

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