The mouse homolog of PKD1: Sequence analysis and alternative splicing

被引：34

作者：

Lohning, C ^{[1
]}

Nowicka, U ^{[1
]}

Frischauf, AM ^{[1
]}

机构：

[1] IMPERIAL CANC RES FUND,LINCOLNS INN FIELDS,LONDON WC2A 3PX,ENGLAND

来源：

MAMMALIAN GENOME | 1997年 / 8卷 / 05期

关键词：

D O I：

10.1007/s003359900429

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned and sequenced the mouse transcript homologous to human polycystic kidney disease 1 (PKD1). The predicted protein is 79% identical to human PKD1 and shows the presence of most of the domains identified in the human sequence. Since the mouse homolog is transcribed from a unique gene and there are no transcribed, closely related copies as has been observed for human PKD1, we have been able to investigate alternative splicing of the transcript. At the junction of exons 12 and 13, several different splicing variants lead to a predicted protein that would be secreted. These forms are predominantly found in newborn brain, while in kidney the transcript homologous to the previously described human RNA predominates.

引用

页码：307 / 311

页数：5

共 18 条

[1]

*AM PKD1 CONS, 1995, HUM MOL GENET, V4, P575

[2]

CLAROS MG, 1994, COMPUT APPL BIOSCI, V10, P685

[3]

DUAN DSR, 1992, J BIOL CHEM, V267, P16076

[4]

*EUR POL KIDN DIS, 1994, CELL, V77, P881

[5] THE POLYCYSTIC KIDNEY-DISEASE-1 (PKD1) GENE ENCODES A NOVEL PROTEIN WITH MULTIPLE CELL RECOGNITION DOMAINS [J].

HUGHES, J ;

WARD, CJ ;

PERAL, B ;

ASPINWALL, R ;

CLARK, K ;

SANMILLAN, JL ;

GAMBLE, V ;

HARRIS, PC .

NATURE GENETICS, 1995, 10 (02) :151-160

[6]

*INT POL KIDN DIS, 1995, CELL, V81, P289

[7] AT LEAST 6 NUCLEOTIDES PRECEDING THE AUG INITIATOR CODON ENHANCE TRANSLATION IN MAMMALIAN-CELLS [J].

KOZAK, M .

JOURNAL OF MOLECULAR BIOLOGY, 1987, 196 (04) :947-950

[8] PKD2, a gene for polycystic kidney disease that encodes an integral membrane protein [J].

Mochizuki, T ;

Wu, GQ ;

Hayashi, T ;

Xenophontos, SL ;

Veldhuisen, B ;

Saris, JJ ;

Reynolds, DM ;

Cai, YQ ;

Gabow, PA ;

Pierides, A ;

Kimberling, WJ ;

Breuning, MH ;

Deltas, CC ;

Peters, DJM ;

Somlo, S .

SCIENCE, 1996, 272 (5266) :1339-1342

[9] THE MURINE INTERLEUKIN-4 RECEPTOR - MOLECULAR-CLONING AND CHARACTERIZATION OF SECRETED AND MEMBRANE-BOUND FORMS [J].

MOSLEY, B ;

BECKMANN, MP ;

MARCH, CJ ;

IDZERDA, RL ;

GIMPEL, SD ;

VANDENBOS, T ;

FRIEND, D ;

ALPERT, A ;

ANDERSON, D ;

JACKSON, J ;

WIGNALL, JM ;

SMITH, C ;

GALLIS, B ;

SIMS, JE ;

URDAL, D ;

WIDMER, MB ;

COSMAN, D ;

PARK, LS .

CELL, 1989, 59 (02) :335-348

[10] The sea urchin sperm receptor for egg jelly is a modular protein with extensive homology to the human polycystic kidney disease protein, PKD1 [J].

Moy, GW ;

Mendoza, LM ;

Schulz, JR ;

Swanson, WJ ;

Glabe, CG ;

Vacquier, VD .

JOURNAL OF CELL BIOLOGY, 1996, 133 (04) :809-817

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