The Use of Fibrous, Supramolecular Membranes and Human Tubular Cells for Renal Epithelial Tissue Engineering: Towards a Suitable Membrane for a Bioartificial Kidney

被引：35

作者：

Dankers, Patricia Y. W. ^{[1
]}

Boomker, Jasper M. ^{[1
]}

Huizinga-van der Vlag, Ali ^{[1
]}

Smedts, Frank M. M. ^{[2
]}

Harmsen, Martin C. ^{[1
]}

van Luyn, Marja J. A. ^{[1
]}

机构：

[1] Univ Groningen, Univ Med Ctr Groningen, Dept Pathol & Med Biol, NL-9713 GZ Groningen, Netherlands

[2] Fdn Collaborating Hosp Eastern Groningen, Pathol Lab, NL-9675 HJ Winschoten, Netherlands

来源：

MACROMOLECULAR BIOSCIENCE | 2010年 / 10卷 / 11期

关键词：

bioartificial kidney; biological application of polymers; hydrogen bonding; membranes; supramolecular structures; tissue engineering; UREMIC ANIMALS; TRANSPORT; DEVICE; POLYMERS; GRADIENT; CULTURE; REPLACEMENT;

D O I：

10.1002/mabi.201000146

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A bioartificial kidney, which is composed of a membrane cartridge with renal epithelial cells, can substitute important kidney functions in patients with renal failure. A particular challenge is the maintenance of monolayer integrity and specialized renal epithelial cell functions ex vivo. We hypothesized that this can be improved by electro-spun, supramolecular polymer membranes which show clear benefits in ease of processability. We found that after 7 d, in comparison to conventional microporous membranes, renal tubular cells cultured on top of our fibrous supramolecular membranes formed polarized monolayers, which is prerequisite for a well-functioning bioartificial kidney. In future, these supramolecular membranes allow for incorporation of peptides that may increase cell function even further.

引用

页码：1345 / 1354

页数：10

共 28 条

[1]

AEBISCHER P, 1987, LIFE SUPPORT SYST, V5, P159

[2] Supramolecular polymers: From scientific curiosity to technological reality [J].