Molecular cloning of a levocabastine-sensitive neurotensin binding site

被引：240

作者：

Chalon, P ^{[1
]}

Vita, N ^{[1
]}

Kaghad, M ^{[1
]}

Guillemot, M ^{[1
]}

Bonnin, J ^{[1
]}

Delpech, B ^{[1
]}

LeFur, G ^{[1
]}

Ferrara, P ^{[1
]}

Caput, D ^{[1
]}

机构：

[1] SANOFI RECH,F-31676 LABEGE,FRANCE

来源：

FEBS LETTERS | 1996年 / 386卷 / 2-3期

关键词：

neurotensin receptor; molecular cloning;

D O I：

10.1016/0014-5793(96)00397-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A search for sequences homologous to the neurotensin receptor cDNA in a rat hypothalamic library has identified a novel neurotensin receptor (NTR-2). The 1539 bp cDNA encodes a 416 amino acid protein and shows highest homology to the previously cloned neurotensin receptor (NTR-1) (64% homology and 43% identity), Binding and pharmacological studies demonstrate that NTR-2 expressed in COS cells recognizes neurotensin (NT) with high affinity as well as several other agonists and antagonists, However, a fundamental difference was found; unlike NTR-1, NTR-2 recognizes, with high affinity, levocabastine, a histamine H1 receptor antagonist previously shown to compete with NT for low-affinity binding sites in brain.

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收藏

页码：91 / 94

页数：4

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