Polyacrylamide containing sugar residues: synthesis, characterization and cell compatibility studies

Hydrophilic polyacrylamide compounds having glucose (PAAm-glucose) and galactose (PAAm-galactose) as pendent groups were synthesized from poly(acryloyl chloride) by polymer analogous reactions. The degree of glucose and galactose substitution was 53.2 and 41.6%, respectively. Tissue culture polystyrene (TCPS) plates coated with these polymers showed increased surface wettability. FTIR-ATR spectra of coated plates showed characteristic bands at 1658, 1716 and 1734 cm(-1) due to the carbonyl groups of polyacrylamide, PAAm-glucose and PAAm-galactose, respectively. Coating of polyacrylamide, PAAm-glucose and PAAm-galactose onto TCPS plates was also confirmed by X-ray photoelectron spectroscopic (XPS) characterization. Rat hepatocytes in primary culture attached to the surfaces of PAAm-glucose and PAAm-galactose, but did not attach to those of polyacrylamide (PAAm). Only small differences were found between PAAm-glucose, PAAm-galactose and PAAm in the attachment of L929 and CHO-K1 cells. Cell growth rate behavior of the latter two cell lines on PAAm-glucose, PAAm-galactose and PAAm was almost identical. (C) 1998 Elsevier Science Ltd. All rights reserved.