A simple and rapid molecular method for Leptospira species identification

被引：11

作者：

Ahmed, Ahmed ^{[1
,2
]}

Anthony, Richard M. ^{[3
]}

Hartskeerl, Rudy A. ^{[1
,2
]}

机构：

[1] WHO FAO OIE, NL-1105 AZ Amsterdam, Netherlands

[2] Royal Trop Inst KIT, Natl Collaborating Ctr Reference & Res Leptospiro, Dept Biomed Res, NL-1105 AZ Amsterdam, Netherlands

[3] Royal Trop Inst KIT, TB Res Unit, Dept Biomed Res, NL-1105 AZ Amsterdam, Netherlands

来源：

INFECTION GENETICS AND EVOLUTION | 2010年 / 10卷 / 07期

关键词：

Leptospira; Molecular; Typing; Micro-array; Ligation; Speciation; REAL-TIME PCR; SP NOV; FAMILY LEPTOSPIRACEAE; DNA HYBRIDIZATION; SEQUENCE-ANALYSIS; GENUS LEPTOSPIRA; INTERROGANS; SEROVARS; BORGPETERSENII; ICTEROHAEMORRHAGIAE;

D O I：

10.1016/j.meegid.2010.06.002

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Serological and DNA-based classification systems only have little correlation. Currently serological and molecular methods for characterizing Leptospira are complex and costly restricting their world-wide distribution and use. Ligation mediated amplification combined with microarray analysis avoidsmany of these drawbacks. We demonstrated that this approach used in the Check-Points (CP) assay can successfully applied for the generic detection of Leptospira and can discriminate between saprophytic, intermediate and pathogenic species. In addition, the CP assay could unambiguously detect strains of seven pathogenic species and revealed discrepancies in previous speciation and culture collections. The method provides a valuable tool adding to the molecular study of leptospires and their local and global distribution. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：955 / 962

页数：8

共 44 条

[1] Development and Validation of a Real-Time PCR for Detection of Pathogenic Leptospira Species in Clinical Materials [J].

Ahmed, Ahmed ;

Engelberts, Mirjam F. M. ;

Boer, Kimberly R. ;

Ahmed, Niyaz ;

Hartskeerl, Rudy A. .

PLOS ONE, 2009, 4 (09)

[2] Multilocus sequence typing method for identification and genotypic classification of pathogenic Leptospira species [J].

Ahmed N. ;

Manjulata Devi S. ;

de los Á Valverde M. ;

Vijayachari P. ;

Machang'u R.S. ;

Ellis W.A. ;

Hartskeerl R.A. .

Annals of Clinical Microbiology and Antimicrobials, 5 (1)

[3]

ALEX JC, 1993, INDIAN J MED MICROBI, V17, P269

[4] Development of multiplex assay for rapid characterization of Mycobacterium tuberculosis [J].

Bergal, I. L. ;

Vijzelaar, R. N. C. P. ;

Costa, E. R. Dalla ;

Schuiterna, A. R. J. ;

Oskam, L. ;

Kritski, A. L. ;

Klatser, P. R. ;

Anthony, R. M. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (02) :689-699

[5] Further determination of DNA relatedness between serogroups and serovars in the family Leptospiraceae with a proposal for Leptospira alexanderi sp. nov. and four new Leptospira genomospecies [J].

Brenner, DJ ;

Kaufmann, AF ;

Sulzer, KR ;

Steigerwalt, AG ;

Rogers, FC ;

Weyant, RS .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :839-858

[6] Monitoring Leptospira Strain Collections: The Need for Quality Control [J].

Cerqueira, Gustavo M. ;

McBride, Alan J. A. ;

Queiroz, Adriano ;

Pinto, Luciano S. ;

Silva, Everton E. ;

Hartskeerl, Rudy A. ;

Reis, Mitermayer G. ;

Ko, Albert I. ;

Dellagostin, Odir A. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2010, 82 (01) :83-87

[7] Distribution of the leptospiral immunoglobulin-like (lig) genes in pathogenic Leptospira species and application of ligB to typing leptospiral isolates [J].

Cerqueira, Gustavo M. ;

McBride, Alan J. A. ;

Picardeau, Mathieu ;

Ribeiro, Samuel G. ;

Moreira, Angela N. ;

Morel, Viviane ;

Reis, Mitermayer G. ;

Ko, Albert I. ;

Dellagostin, Odir A. .

JOURNAL OF MEDICAL MICROBIOLOGY, 2009, 58 (09) :1173-1181

[8] A century of Leptospira strain typing [J].

Cerqueira, Gustavo M. ;

Picardeau, Mathieu .

INFECTION GENETICS AND EVOLUTION, 2009, 9 (05) :760-768

[9]

CINCO M, 1989, FEMS (Federation of European Microbiological Societies) Microbiology Immunology, V47, P499, DOI 10.1111/j.1574-6968.1989.tb02441.x

[10]

ELLINGHAUSEN HC, 1965, AM J VET RES, V26, P45

← 1 2 3 4 5 →