Biological cryo-atomic force microscopy: Instrumentation and applications

被引:6
作者
Sheng, ST [1 ]
Shao, ZF [1 ]
机构
[1] Univ Virginia, Sch Med, Dept Mol Physiol & Biol Phys, Charlottesville, VA 22908 USA
来源
JAPANESE JOURNAL OF APPLIED PHYSICS PART 1-REGULAR PAPERS BRIEF COMMUNICATIONS & REVIEW PAPERS | 1998年 / 37卷 / 6B期
关键词
atomic force microscopy; low temperature; biology; molecular structure;
D O I
10.1143/JJAP.37.3828
中图分类号
O59 [应用物理学];
学科分类号
摘要
Although atomic force microscopy (AFM) has been successfully applied to a number of biological specimens under aqueous solution, its resolving power has been limited due to specimen deformation or damage induced by the scanning tip, especially with large flexible and heterogeneous specimens. To further expand the capabilities of the AFM, a cryo-AFM was recently developed to operate under ambient pressure in liquid nitrogen vapor. The operating principle of this novel system is discussed, and some of the most recent applications of the cryo-AFM to various biological specimens are reviewed. These results have clearly demonstrated the power of the cryo-AFM in the study of biological structures, and at the same time, have revealed novel structural information of several important molecules.
引用
收藏
页码:3828 / 3833
页数:6
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