Glucagon production of the rat insulinoma cell line INS-1 -: A quantitative comparison with primary rat pancreatic islets

被引：18

作者：

Bollheimer, LC ^{[1
]}

Wrede, CE ^{[1
]}

Rockmann, F ^{[1
]}

Ottinger, I ^{[1
]}

Schölmerich, J ^{[1
]}

Buettner, R ^{[1
]}

机构：

[1] Univ Regensburg, Dept Internal Med 1, D-93042 Regensburg, Germany

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 330卷 / 01期

关键词：

INS-1; insulinoma; islet; glucagon; preproglucagon-mRNA; free fatty acids;

D O I：

10.1016/j.bbrc.2005.01.168

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The rat insulinoma cell line INS-1 is the most commonly used clonal cell model in pancreatic beta-cell research. Considering the multihormonality of many insulinomas we examined as to how INS-1 cells comply with the notion of resembling a pure P-cell line. Glucagon immunoassays revealed that INS-1 cells secrete glucagon in a similar range as islets. By immunohistochemistry we detected a cytoplasmic glucagon signal in INS-1 cells which colocalized with C-peptide. Cellular content of preproglucagon-mRNA and glucagon protein in INS-1 cells was less than two percent of the respective values in islets, which probably reflects differences in the intracellular metabolism and/or secretory pathways. Taken together, it is obvious that INS-1 cells do not represent an exclusively insulin producing beta-cell line. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：327 / 332

页数：6

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