Receptor-targeted optical imaging of tumors with near-infrared fluorescent ligands

被引:419
作者
Becker, A
Hessenius, C
Licha, K
Ebert, B
Sukowski, U
Semmler, W
Wiedenmann, B
Grötzinger, C
机构
[1] Humboldt Univ, Klinikum Charite, Med Klin Hepatol & Gastroenterol, D-13353 Berlin, Germany
[2] Free Univ Berlin, Inst Diagnost Forsch GMBH, D-14050 Berlin, Germany
[3] Deutsch Krebsforschungszentrum, D-69120 Heidelberg, Germany
关键词
D O I
10.1038/86707
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
We report here the in vivo diagnostic use of a peptide-dye conjugate consisting of a cyanine dye and the somatostatin analog octreotate as a contrast agent for optical tumor imaging. When used in whole-body in vivo imaging of mouse xenografts, indotricarbocyanine-octreotate accumulated in tumor tissue. Tumor fluorescence rapidly increased and was more than threefold higher than that of normal tissue from 3 to 24 h after application. The targeting conjugate was also specifically internalized by primary human neuroendocrine tumor cells. This imaging approach, combining the specificity of ligand/receptor interaction with near-infrared fluorescence detection, may be applied in various other fields of cancer diagnosis.
引用
收藏
页码:327 / 331
页数:5
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